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<h2>Registered report: Intestinal inflammation targets cancer-inducing activity of the microbiota</h2> <p><br> <strong>Abstract:</strong></p> <p>The <a href="https://osf.io/e81xl/wiki/home/" rel="nofollow">Reproducibility Project: Cancer Biology</a> seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Intestinal Inflammation Targets Cancer-Inducing Activity of the Microbiota’ by <a href="https://doi.org/10.1126/science.1224820" rel="nofollow">Arthur et al. (2012)</a>, published in <em>Science</em> in 2012. Arthur and colleagues identified a genotoxic island in <em>Escherichia coli</em> NC101 that appeared to be responsible for causing neoplastic lesions in inflammation-induced <em>IL10<sup>−/−</sup></em> mice treated with azoxymethane. The experiments that will be replicated are those reported in Figure 4 <a href="https://doi.org/10.1126/science.1224820" rel="nofollow">(Arthur et al., 2012)</a>. Arthur and colleagues inoculated <em>IL10<sup>−/−</sup></em> mice with a mutated strain of <em>E. coli</em> NC101 lacking the genotoxic island, and showed that those mice suffered from fewer neoplastic lesions than mice inoculated with the wild type form of <em>E. coli</em> NC101 (Figure 4). The Reproducibility Project: Cancer Biology is a collaboration between the <a href="https://cos.io/" rel="nofollow">Center for Open Science</a> and <a href="https://www.scienceexchange.com/" rel="nofollow">Science Exchange</a>, and the results of the replications will be published by <a href="https://elifesciences.org/" rel="nofollow"><em>eLife</em></a>.</p> <hr> <h3>Contents</h3> <p>This component contains additional materials relating to the Registered Report for Study 41: Arthur et al., 2012 (<em>Science</em>).</p> <p>The published Registered Report can be found <a href="http://elifesciences.org/content/4/e04186" rel="nofollow">here</a>.</p> <p><strong>Registered Report Initial Draft</strong>:</p> <ul> <li>This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input.<ul> <li><a href="https://osf.io/7kph5/" rel="nofollow">Study 41 Registered Report Initial Draft</a></li> </ul> </li> </ul> <p><strong>Power Calculations</strong>:</p> <ul> <li>These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used.<ul> <li><a href="https://osf.io/f4iuy/" rel="nofollow">Study 41 power calculations</a></li> <li><a href="https://osf.io/84qui/" rel="nofollow">Study 41 Figure 4E data estimates</a></li> </ul> </li> </ul>
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