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<p>Target of replication: <em>A priori</em> experimental protocols, materials, and confirmatory analysis plans were peer reviewed, <a href="https://osf.io/rvyg5/" rel="nofollow">pre-registered</a>, and published by <a href="https://elifesciences.org/content/3/e04180" rel="nofollow"><em>eLife</em></a>.</p> <p>This component includes all primary and QC data for the replication of <a href="http://www.nature.com/nature/journal/v485/n7399/full/nature11071.html" rel="nofollow">Berger et al. 2012</a>. This experiment was performed to assess the genotype of endogenous <em>PREX2</em> in NRAS<sup>G12D</sup> melanocytes. </p> <h3>Contents</h3> <p><a href="https://osf.io/rgcxn/wiki/home/" rel="nofollow">Figures component</a> contains a visualization of the results. These figures are the same as can be found in the Replication Study and include Figure 1 displaying the sequencing results. </p> <p><a href="https://osf.io/vsxed/wiki/home/" rel="nofollow">Data Component</a>, contains data generated from the experimental work. The <a href="https://osf.io/vsxed/files/" rel="nofollow">PREX2 Sequencing Second Attempt (TACGen)</a> folder containts raw data from the replicating lab (.ab1 files and sequence calls in text format (.seq)) which successfully sequenced the endogenous <em>PREX2</em> from the NRAS<sup>G12D</sup> cell line reported in the Replication Study. The <a href="https://osf.io/vsxed/files/" rel="nofollow">PREX2 Sequencing First Attempt (Biofactura)</a> folder contains gel pictures and summary .pptx files from a failed attempt to sequence the <em>PREX2</em> gene. </p> <p><a href="https://osf.io/73a2w/wiki/home/" rel="nofollow">Methods Component</a>, contains methodology details for this replication attempt which were briefly described in the <a href="https://elifesciences.org/content/3/e04180" rel="nofollow">Registered Report</a>.</p> <p><a href="https://osf.io/38wq2/wiki/home/" rel="nofollow">Cell line quality Control data</a> includes results confirming the cell line was free of mycoplasma contamination as well as STR DNA profiling.</p>
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