![enter image description here][1] Welcome to the **2019 DNA Barcoding for CUREs Workshop** organized & presented by educators from [James Madison University (JMU)][3], Cold Spring Harbor Laboratory’s [DNA Learning Center (DNALC)][4], [New York City College of Technology (City Tech)][5], and [Bowie State University (BSU)][6]. This workshop is supported by [NSF IUSE Grant #1821657][7]. ![enter image description here][8] Use the Wiki links in the left pane to find info and activities for each day. **General Workshop Info** **Location**: The workshop will be held in the Biosciences bldg on the campus of James Madison University in Harrisonburg, VA. See below for classroom and hotel details. **Workshop Computing requirements**: You will need to bring your own Wi-Fi enabled personal laptop to the workshop. A limited number of loaner laptops (macs) are available, please let the organizers know ASAP if you need a loaner. We will also be using the iNaturalist database and smart phone app. To actively participate in the workshop you will need to download/register for the following: **Downloads:** - Latest version of Firefox: https://www.mozilla.org/en-US/ or Chrome web browsers: https://www.google.com/chrome/browser/desktop/ **Free registrations:** - DNA Subway/CyVerse account: https://dnasubway.cyverse.org/ - Please ensure that you can log onto DNA Subway - Open Science Framework (OSF) account: https://osf.io/ - How to make an OSF account tutorial http://help.osf.io/m/account/l/696112-create-an-osf-account - Please ensure that you can view the workshop OSF workspace: https://osf.io/qtesm/wiki/home/ - iNaturalist account: https://www.inaturalist.org/ - Please ensure that you can log onto iNaturalist - Smartphone with iNaturalist mobile app downloaded and installed: https://itunes.apple.com/us/app/inaturalist/id421397028?mt=8 - Please ensure you can open the iNaturalist app on your phone **Wireless Access for Non-JMU attendees** (case sensitive): Network: JMU-Visitors Username: festivalsummer Password: 168693 **Other Workshop Info** **Lodging:** Rooms and hotel parking will be provided at [Hotel Madison][9] (710 S. Main Street Harrisonburg, VA 22801, 540-564-0200) which is an ~25 minute walk or 10 minute drive from the workshop location in the JMU Biosciences building. Travel to/from the hotel can be found [here][10]. Hotel check in is available ≥4pm Sunday afternoon and check out will be Friday morning before 11am. Attendees are responsible for travel from the Hotel to the workshop location. [Here are the driving directions][11] and [here are the walking directions][12] to/from the JMU Biosciences building. **Workshop Parking:** Lot D2, directly across from the Biosciences bldg at 951 Carrier Drive is the designated visitor parking lot for the workshop. Workshop participants will be issued parking permits which will be required for visitor parking in this lot on the workshop dates. **Food:** Breakfast vouchers for the Madison Hotel restaurant and on campus lunchs will be provided for all registered workshop attendees. Both venues offer diverse dining options that fit most dietary restrictions. Coffee and snack breaks will be provided during the workshop. There are snack/beverage vending machines in the Biosciences bldg. There is also a Starbucks coffee shop in the nearby Rose Library that is open from 8am-4pm Mon-Thur and 9am-noon on Friday. Attendees will be responsible for dinners. The Madison Hotel restaurant serves dinner and there are also a number of mixed dinning options within walking/short driving distance in the downtown area. [Here are directions from the hotel to the downtown area][13] (~10 min 0.5 mile walk). **Attire:** Part of the first day of the workshop will be spent in the JMU arboretum, rain or shine. Please bring a rain jacket and appropriate attire for a light hike. **Ground travel from airports to Harrisonburg:** - [Shuttle from Shenandoah Regional Airport (SHD)][14] - [Shuttle options from Charlottesville Albermarle (CHO) and Dulles International (IAD)][15] If you have any additional questions, please email Ray Enke (enkera@jmu.edu), Ollie Hyman (hymanoj@jmu.edu), or Bruce Nash (nash@cshl.edu) Thanks, and looking forward to working with you all! **Other DNA Barcoding and Blue Line resources:** - DNA Barcoding 101: https://www.dnabarcoding101.org/ - BOLD (Barcode Of Life Data System): http://www.boldsystems.org/ - Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC522015/ - A botanical macroscope: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722277/ - DNA barcoding Brooklyn (New York): A first assessment of biodiversity in Marine Park by citizen scientists: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199015 - MUSCLE (MUltiple Sequence Comparison by Log- Expectation): https://www.ebi.ac.uk/Tools/msa/muscle/ - PHYLIP (the PHYLogeny Inference Package): http://evolution.genetics.washington.edu/phylip/general.html ![enter image description here][16] [1]: https://mfr.osf.io/export?url=https://osf.io/7aph4/?action=download&mode=render&direct&public_file=True&initialWidth=647&childId=mfrIframe&parentTitle=OSF%20%7C%20Groupshot2019.jpg&parentUrl=https://osf.io/7aph4/&format=2400x2400.jpeg [2]: https://mfr.osf.io/export?url=https://osf.io/xw64u/?action=download&mode=render&direct&public_file=True&initialWidth=647&childId=mfrIframe&parentTitle=OSF%20%7C%20workshop.jpg&parentUrl=https://osf.io/xw64u/&format=2400x2400.jpeg [3]: https://www.jmu.edu/biology/ [4]: https://www.dnalc.org/ [5]: https://www.citytech.cuny.edu/biological/ [6]: https://www.bowiestate.edu/academics-research/colleges/college-arts-sciences/departments/natural-sciences/ [7]: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1821657&HistoricalAwards=false [8]: https://mfr.osf.io/export?url=https://osf.io/2r36k/?action=download&direct&mode=render&view_only=867240cbde5d4f8686942ce4ad4b586f&public_file=True&initialWidth=624&childId=mfrIframe&parentTitle=OSF%20%7C%20funding.jpg&parentUrl=https://osf.io/2r36k/?view_only=867240cbde5d4f8686942ce4ad4b586f&format=2400x2400.jpeg [9]: https://www.hotelmadison.com/ [10]: https://www.hotelmadison.com/maps-directions [11]: https://goo.gl/maps/y5r6U8C5cvCrzRWu9 [12]: https://goo.gl/maps/CGzGUkxEXYEKwZYr8 [13]: https://goo.gl/maps/RmrPjLjp7JCRcpQg6 [14]: https://flyshd.com/shd-shuttle-reservation/ [15]: https://www.jmu.edu/transportation/regional-and-break-shuttles/taxi-shuttle.shtml [16]: https://mfr.osf.io/export?url=https://osf.io/2r36k/?action=download&direct&mode=render&view_only=867240cbde5d4f8686942ce4ad4b586f&public_file=True&initialWidth=624&childId=mfrIframe&parentTitle=OSF%20%7C%20funding.jpg&parentUrl=https://osf.io/2r36k/?view_only=867240cbde5d4f8686942ce4ad4b586f&format=2400x2400.jpeg