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**SEE MAIN PROJECT WIKI FOR GENERAL INFORMATION ON STUDY BACKGROUND AND DATA COLLECTION** Primary Hypothesis: AI/AN with more exposure to discrimination will exhibit heightened physiological reactivity and/or slower physiological recovery to a discrimination-based stress task. Overview of procedures: 1) Participants were fitted with with an electronic blood pressure cuff, 3 single electrodes for ECG recording, and 4 dual electrodes for ICG recording. 2) Blood pressure, saliva samples (to measure cortisol and alpha amylase), and mood ratings were taken at specific intervals (see chart); ECG and ICG were continuously recorded. 3) After a 15-minute baseline rest period, participants were asked to complete a simple 30-sec math task (count down by 3's from 100), and then to recount a prior experience of discrimination 4) Recorded instructions: For the next challenge task, you will talk about a past experience in which you knew you were treated poorly or unfairly because you are American Indian, even if the other person didn’t mention it. Please describe an experience that bothered you or stayed with you. You’ll have a couple of minutes to prepare. I want to let you know that once you finish telling your story, the resarch assistant will immediately ask for another saliva sample, and measure your mood and blood-pressure. She won’t be able to talk with you about your experience until your return visit tomorrow. When you talk about your experience, please answer the questions shown on the sheet in front of you <DEST-Handout>: What happened? who were you with? Where were you? What did the other person do or say? How did you feel? What did you do or say? I am now going to give you 2 minutes to think about the event you want to talk about. You will hear a prompt to tell you when to begin talking; the research assistant will then come in for the next task. 5) Once the participant was finished telling their story, they were asked to sit quietly and rest for 30 minutes. BP, saliva, and mood ratings were measured at the beginning and every 15 minutes during this period.
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