07/10/17 | 8:18 PM
Performed mutagenesis reaction another time, since the first did not get the desired bands.
Added 5uL of template DNA
1.25uL of each primer set, this time using pqn-59_GFP_TOP and pqn-59_GFP_BOT primer.
Added 1uL of HF polymerase
Added 5uL of 10X rxn buffer
Added 3uL of DMSO
Performed 11kb QuickChange cycle on PCR machine.
After this, added 1uL of DpnI to the mix and let...