PQN-59 Function  /

Mutagenesis of PQN-59, Removal of GFP

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07/10/17 | 8:18 PM Performed mutagenesis reaction another time, since the first did not get the desired bands. Added 5uL of template DNA 1.25uL of each primer set, this time using pqn-59_GFP_TOP and pqn-59_GFP_BOT primer. Added 1uL of HF polymerase Added 5uL of 10X rxn buffer Added 3uL of DMSO Performed 11kb QuickChange cycle on PCR machine. After this, added 1uL of DpnI to the mix and let...

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