To reproduce the figures, download the individual zip packages in each directory (corresponding to individual experiments); upon unzipping, start the associated .RProject file and run the associated RNotebook (.Rmd).
For any issues with running the analyses or accessing the files, please contact Ralitsa Madsen: rmadsen001@dundee.ac.uk
Exact experimental details are provided as part of the associated manuscript: https://www.biorxiv.org/content/10.1101/2023.12.23.573207v2
Note that the final trusted antibody markers are those included in the "figure_output" directory for each dataset.
- pAKT S473
- pS6 S240/S244
- pNDRG1 T346
- pERK1/2 T202/Y204; T185/Y187
- pSMAD2/3 S465/S467; S423/S425
- pRB S807/S811
- cCASP3 D175
- S6 total
- AKT total
Any other antibodies included in the raw data are not relevant to the publication but were part of ongoing antibody panel optimisations/titrations - they should not be used as they are still undergoing validation.