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<h2>Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET</h2> <p><br> <strong>Abstract</strong> The <a href="https://osf.io/e81xl/wiki/home/" rel="nofollow">Reproducibility Project: Cancer Biology</a> seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of <a href="https://osf.io/e81xl/wiki/studies/" rel="nofollow">citations and Altmetric scores</a> (<a href="https://elifesciences.org/content/3/e04333" rel="nofollow">Errington et al., 2014</a>). This Registered Report describes the proposed replication plan of key experiments from “Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET” by Peinado and colleagues, published in <em>Nature Medicine</em> in 2012 (<a href="http://www.nature.com/articles/nm.2753" rel="nofollow">Peinado et al., 2012</a>). The key experiments being replicated are from Figures 4E, as well as Supplementary Figures 1C and 5A. In these experiments, Peinado and colleagues show tumor exosomes enhance metastasis to bones and lungs, which is diminished by reducing Met expression in exosomes (<a href="http://www.nature.com/articles/nm.2753" rel="nofollow">Peinado et al., 2012</a>). The Reproducibility Project: Cancer Biology is a collaboration between the <a href="https://cos.io/" rel="nofollow">Center for Open Science</a> and <a href="https://www.scienceexchange.com/" rel="nofollow">Science Exchange</a> and the results of the replications will be published in <a href="https://elifesciences.org/" rel="nofollow"><em>eLife</em></a>.</p> <hr> <h3>Contents</h3> <p>This component contains additional materials relating to the Registered Report for Study 42: Peinado et al. 2012, <em>Nature Medicine</em>.</p> <p>The Registered Report can be found <a href="http://elifesciences.org/content/5/e07383" rel="nofollow">here</a>.</p> <p><strong>Registered Report Initial Draft</strong>:</p> <ul> <li>This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input.<ul> <li><a href="https://osf.io/g8avb/" rel="nofollow">Study 42 Registered Report Initial Draft</a></li> </ul> </li> </ul> <p><strong>Power Calculations</strong>:</p> <ul> <li>These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used.<ul> <li><a href="https://osf.io/6xw7z/" rel="nofollow">Study 42 Power Calculations</a></li> <li><a href="https://osf.io/gny47/" rel="nofollow">Study 42 Protocol 4 1-way ANOVA and Kruskal-Wallis test.R</a></li> </ul> </li> </ul>
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