**Figure 2. Expression of PREX2 isoforms.** NRAS^G12D melanocytes were transduced to express GFP or PREX2 isoforms (PREX2^WT, PREX2^G844D, PREX2^Q1430*). (**A**) Representative Western blot using an anti-V5 antibody (top panel) or an anti-alpha-Tubulin antibody (bottom panel). Lanes 1-4 ɑre from cells transduced with GFP, PREX2^WT, PREX2^G844D, or PREX2^Q1430*, respectively, generated during the original study ([Berger et al., 2012][1]). Lanes 5-8 are from cells transduced with GFP, PREX2^WT, PREX2^G844D, or PREX2^Q1430*, respectively, made during this replication attempt (RP:CB). Membranes were cut at ~70 kDa so that V5 and alpha-Tubulin could be probed in parallel. (**B**) Relative protein expression (V5/Tubulin) of ectopically expressed PREX2 isoforms are presented for each stable cell line and normalized to PREX2^WT cells made during the original study. Means represented by red bars, circles indicate individual biological replicates [n=3], and error bars indicate 95% CI. Green circles represent protein expression reported in Figure S6A of the original study. Two-way ANOVA main effect of PREX2 isoform (*F*(2,12) = 4.608, *p* = .033), the main effect of study (*F*(1,12) = 8.701, *p* = .012), and isoform:study interaction (*F*(2,12) = 7.52, *p* = .008). (**C**) Representative Western blot using an anti-PREX2 antibody (top panel) or an anti-alpha-Tubulin antibody (bottom panel). Lanes 1-4 are from cells generated during the original study transduced with GFP, PREX2^WT, PREX2^G844D, or PREX2^Q1430*,respectively. Lanes 5-8 are from cells made during this replication attempt transduced with GFP, PREX2^WT, PREX2^G844D, or PREX2^Q1430*, respectively. Membranes were cut at ~70 kDa so that PREX2 and alpha-Tubulin could be probed in parallel. (**D**) Relative protein expression (PREX2/Tubulin) of ectopically expressed PREX2 isoforms are presented for each stable cell line and normalized to PREX2^WT cells generated during the original study. Means represented by red bars, circles indicate individual biological replicates [n=3], and error bars indicate 95% CI. Two-way ANOVA main effect of PREX2 expression (*F*(3,16) = 15.033, *p* = 6.47x10^-5), the main effect of study (*F*(1,16) = 13.08, *p* = .002), expression:study interaction (*F*(3,16) = 4.50, *p* = .018). Access [full size image][5] and [svg file][6] made with [Inkscape][7], version 0.91. [Figure 2B][8] was generated with this [R script][9] and will call the [data][10] directly from the OSF. [Figure 2D][11] was generated with this [R script][12] and will call the [data][13] directly from the OSF. Representative Western blot images presented in the figure are these files: (**A**) [V5_2_5sec.tif][14], (**C**) [PREX2_1_5sec.tif][15]. All Western blot images can be found [here][16]. ---------- [Statistical Analyses component][17] contains R scripts that were used for the analysis. [1]: http://www.nature.com/nature/journal/v485/n7399/full/nature11071.html [5]: https://osf.io/xmqft/ [6]: https://osf.io/ugf5k/ [7]: https://inkscape.org/en/ [8]: https://osf.io/3fm9y/ [9]: https://osf.io/qehyv/ [10]: https://osf.io/3kuec/ [11]: https://osf.io/uqrv4/ [12]: https://osf.io/fgv5e/ [13]: https://osf.io/6k4zq/ [14]: https://osf.io/g2krv/ [15]: https://osf.io/cbhmt/ [16]: https://osf.io/nm2jv/files/ [17]: https://osf.io/w52c7/wiki/home/