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Description: The current project examines, through two independent experiments, the role of neuroticism on evaluative conditioning. Evaluative conditioning (EC) is an effect which consists in repeatedly presentations of a conditioned stimulus (CS) with a positive or negative unconditioned stimulus (US), resulting a valence transfer from the US to the CS. To further investigate the interindividual differences of neuroticism on this effect, we introduced the uncertainty/ ambivalence element which could help us to capture the natural tendency of highly neurotic people in transferring negative valence. Experiment 1 presented an experimental manipulation at the US level by using ambivalent USs (i.e., a positive picture and a negative picture merged into one image), whereas Experiment 2 provided a reinforcement manipulation by presenting two CSs with positive USs in half of presentations and with negative USs in other half of presentations. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the grant agreement No. 952464.