**Figure 2. Overall survival in JQ1-treated MM.1S-luc orthotopic xenograft model.** Kaplan-Meier plot of overall survival during the pre-specified study period of 36 days. Female SCID-beige mice with established MM.1S-luc orthotopic xenografts were randomized to daily IP injections of 50 mg/kg (+)-JQ1, 50 mg/kg (-)-JQ1, or vehicle control. Total number of mice (n= 12 for each group). Log-rank (Mantel-Cox) test of (+)-JQ1 treatment compared to vehicle control; uncorrected p = .024, a priori Bonferroni adjusted alpha level = .025; (Bonferroni corrected p = .047). Log-rank (Mantel-Cox) test of (+)-JQ1 compared to (-)-JQ1; uncorrected p = .093, a priori Bonferroni adjusted alpha level = .025; (Bonferroni corrected p = .187). Access the [full size image][1] and [R script][2] used to generate this figure. This script will call the data directly from the OSF. **Figure 3. Tumor burden in JQ1-treated MM.1S-luc orthotopic xenograft model.** Female SCID-beige mice were orthotopically xenografted after intravenous injection with MM.1S-luc cells. Following detection of established disease (diffusely engrafted in the skeleton with an increase in bioluminescence), mice were randomly assigned to receive daily IP injections of 50 mg/kg (+)-JQ1, 50 mg/kg (-)-JQ1, or vehicle control (VEH). (**A**) Representative whole-body bioluminescence images of mice bearing MM.1S tumors 22 days after the start of the indicated treatment. (**B**) Line graph of tumor burden, as measured by whole-body bioluminescence, of tumor bearing mice during the course of the indicated treatment. Means reported and error bars represent s.e.m. The number of mice per condition at start of treatment (n=12 for each group) and at day 22 ((+)-JQ1 = 11, (-)-JQ1 = 9, Vehicle = 7). One-way ANOVA on a natural log transformed day 22 bioluminescence signal; *F*(2,24) = 1.126, *p* =.341. The pairwise contrast between (+)-JQ1 treatment and Vehicle (Fisher’s LSD test); *t*(24) = 1.303, *p* = .205 with *a priori* alpha level = .05. The pairwise contrast between (+)-JQ1 and (-)-JQ1 treatment (Fisher’s LSD test); *t*(24) = 1.221, *p* = .234 with a priori alpha level = .05. Access the [full size image][3] and [svg file][4] made with [Inkscape][5], version 0.91. [Figure 3B][6] was generated with this [R script][7] and will call the data directly from the OSF. Representative IVIS images are: vehicle control treated mice [342,311,339][8]; (+)-JQ1 treated mice [324,326,343][9]; (-)-JQ1 treated mice [331,338,341][10]. All experimental IVIS images can be found [here][11]. **Figure 3 - figure supplement 1.** Individual tumor xenografts. This is the same experiment as in Figure 3, but with the bioluminescence data plotted on a continuous scale (**A**) or natural log transformed scale (**B**) for each animal rather than averages. Female SCID-beige mice with established MM.1S-luc orthotopic xenografts were randomly assigned to receive daily IP injections of 50 mg/kg (+)-JQ1, (-)-JQ1, or vehicle control. The number of mice per condition at start of treatment (n=12 for each group) and at day 22 ((+)-JQ1 = 11, (-)-JQ1 = 9, Vehicle = 7). Access the [full size image][12] and [R script][13] used to generate this figure. This script will call the data directly from the OSF. ---------- [Statistical Analyses component][14] contains R scripts that were used for the analysis. [1]: https://osf.io/nckxg/ [2]: https://osf.io/jvye3/ [3]: https://osf.io/7ugf6/ [4]: https://osf.io/f46hm/ [5]: https://inkscape.org/en/ [6]: https://osf.io/ajbmc/ [7]: https://osf.io/u6s7n/ [8]: https://osf.io/kneyb/ [9]: https://osf.io/3ndjx/ [10]: https://osf.io/yp925/ [11]: https://osf.io/jse9q/files/ [12]: https://osf.io/zkgm5/ [13]: https://osf.io/dkxsz/ [14]: https://osf.io/yznr4/wiki/home/