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This project contains all information pertaining to the replication of experiments from “Diverse somatic mutation patterns and pathway alterations in human cancers” published in *Nature* in 2010 ([Kan et al., 2010][1]). Experimental work began although not all experiments were completed. The completed experiments were included in the summary paper "Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology" ([Errington et al., 2021][2]). <br> Also, explore the Registered Report and materials related to the Registered Report [here][3]. <br> **Data and Material Availability**: All other associated data, protocols, analysis scripts, and other digital materials are available within this OSF project. **Experiments replicated**: Reproduce and explore the figures, analyses, data, and methods generated in this replication attempt. - [Anchorage-independent colony formation assay of HMECs transduced with wild-type or mutant GNAO1][4] - Replication of Figures 3D-F of Kan et al., 2010 - [Preliminary results of other experiments described in the Registered Report][5] - Attempt of replication of Figures 4C-F of Kan et al., 2010 **Meta-analysis**: As a measure of evaluating reproducibility a meta-analysis of each effect was performed. The forest plots, analyses, and data are available [here][6]. <br> Questions about the project can be directed to contact+rpcb@cos.io. [1]: https://doi.org/10.1038/nature09208 [2]: https://doi.org/10.7554/eLife.73430 [3]: https://elifesciences.org/articles/11566 [4]: https://osf.io/kcaez/wiki/home [5]: https://osf.io/s647k/wiki/home [6]: https://osf.io/a4uym/wiki/home