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Date created: 2021-08-03 11:01 AM | Last Updated: 2023-04-01 10:02 AM

Category: Project

Description: This project contains all TIRF imaging data and analyses scripts necessary to reproduce the relevant figures in Gong et al. 2023 Nature.

License: CC-By Attribution 4.0 International

Wiki

Total internal fluorescence (TIRF) microscopy of phosphoinositide reporters

TIRF microscopy which allows selective imaging of the small cell volume, including the plasma membrane, directly adjacent to the coverslip onto whih cells have been seeded. HeLa or A549 cells were seeded in Matrigel-coated (Corning #354230; diluted in Opti-MEM at 1:50) 8-well chamber slides (glass bottom, 1.55 refractive i…

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Dropbox: SD869706_A017_31082020_A549
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Dropbox: SD927953_2020-10-10-HeLa_PIP3_Pax_1938_BYL
OSF Storage (Germany - Frankfurt)
Dropbox: SD932847_2020-10-14_HeLa_PI34P2_Pax_1938_BYL
OSF Storage (Germany - Frankfurt)

Citation

Components

HeLa_PIP3_PI34P2_all_combined

Processed values from all HeLa experiments presented in the publication, alongside the scripts used to produce the final summary data illustrated in t...

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SD869706_A017_31082020_A549

Raw & processed data for A549 PIP3 reporter assays, alongside all the necessary scripts to reproduce the published figures.

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SD927953_2020-10-10-HeLa_PIP3_Pax_1938_BYL

Experimental data and associated scripts for HeLa cells used for PIP3 reporter assays.

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SD932847_2020-10-14_HeLa_PI34P2_Pax_1938_BYL

Experimental data and associated scripts for HeLa cells used for PI(3,4)P2 reporter assays, 1/2

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SD940576_2020-10-20_HeLa_PI34P2_1938_BYL

Experimental data and associated scripts for HeLa cells used for PI(3,4)P2 reporter assays, 2/2

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