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Description: Diabetic foot ulcers (DFU) are one of the most common complications for those with poorly controlled diabetes mellitus. chronic infection, hypoxia, hyperglycemia. The depth of the wound, the severity of the infection, and the degree of vascular insufficiency are used to categorize DFUs. This classification system aids in the development of DFU A greater number of individuals are at risk from enterobacteriaceae since they are also challenging to treat and more common. (E. coli): cefepime, ceftazidime, or cefotaxime. To detect the phenotypic characteristics of E. coli, MacConkey with crystal violet agar and (EMB agar were used. Targeting the 16S rDNA oligonucleotide16S rDNA primers was used for the PCR analysis of isolated E. coli. E. coli accounted for about 36.84% of the total isolates, in the study E.coli is the most common species among the bacteria that's causing of DFI from enterobacteriaceae. It was strongly associated with the type of infection and wounds grade. E. coli was present in all isolates and at 100%, and that a molecular weight of 585.