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<p>This project aims to develop low cost and open diagnostics for SARS-CoV-2 based on two main isothermal reactions: RT-LAMP and RPA.</p> <p>This repository will collect data and release up-to-date progress.</p> <h4><strong>Preliminary protocols for enzyme purification for RT, RPA and LAMP</strong></h4> <ul> <li> <p><strong>BstLF</strong>: <a href="https://dx.doi.org/10.17504/protocols.io.bksrkwd6" rel="nofollow">Expression and purification protocol</a>, <a href="https://benchling.com/s/seq-MuNUtRESOLQyYjeRVm8Z" rel="nofollow">genbank seq</a> and <a href="https://forum.reclone.org/t/bst-large-fragment/91/10" rel="nofollow">discussion thread at ReClone</a> </p> </li> <li> <p><strong>MMLV RT</strong>: <a href="https://www.protocols.io/view/recombinant-expression-and-purification-of-codon-o-bhcvj2w6" rel="nofollow">Expression and purification protocol</a>, <a href="https://benchling.com/s/seq-LZOeOlZ90svPCI5vrlQF" rel="nofollow">genbank seq</a> y <a href="https://forum.reclone.org/t/mmlv-reverse-transcriptase/88" rel="nofollow">discussion thread at ReClone</a> </p> </li> </ul> <h4><strong>Preliminary results</strong></h4> <ul> <li><strong>RT:LAMP</strong>: <a href="https://www.protocols.io/edit/low-cost-lamp-and-rt-lamp-bsejnbcn" rel="nofollow">Buffers and reactions were prepared according to these open protocols</a> and <a href="https://github.com/LabTecLibres/RT-LAMP" rel="nofollow">Code for reaction optimization</a>.</li> </ul> <p>All the DNAs are accessible under <a href="https://biobricks.org/openmta/" rel="nofollow">openMTA</a></p>
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