Loading wiki pages...

Wiki Version:
<p>This project aims to develop low cost and open diagnostics for SARS-CoV-2 based on two main isothermal reactions: RT-LAMP and RPA.</p> <p>This repository will collect data and release up-to-date progress.</p> <h4><strong>Preliminary protocols for enzyme purification for RT, RPA and LAMP</strong></h4> <ul> <li> <p><strong>BstLF</strong>: <a href="https://dx.doi.org/10.17504/protocols.io.bksrkwd6" rel="nofollow">Expression and purification protocol</a>, <a href="https://benchling.com/s/seq-MuNUtRESOLQyYjeRVm8Z" rel="nofollow">genbank seq</a> and <a href="https://forum.reclone.org/t/bst-large-fragment/91/10" rel="nofollow">discussion thread at ReClone</a> </p> </li> <li> <p><strong>MMLV RT</strong>: <a href="https://www.protocols.io/view/recombinant-expression-and-purification-of-codon-o-bhcvj2w6" rel="nofollow">Expression and purification protocol</a>, <a href="https://benchling.com/s/seq-LZOeOlZ90svPCI5vrlQF" rel="nofollow">genbank seq</a> y <a href="https://forum.reclone.org/t/mmlv-reverse-transcriptase/88" rel="nofollow">discussion thread at ReClone</a> </p> </li> </ul> <h4><strong>Preliminary results</strong></h4> <ul> <li><strong>RT:LAMP</strong>: <a href="https://www.protocols.io/edit/low-cost-lamp-and-rt-lamp-bsejnbcn" rel="nofollow">Buffers and reactions were prepared according to these open protocols</a> and <a href="https://github.com/LabTecLibres/RT-LAMP" rel="nofollow">Code for reaction optimization</a>.</li> </ul> <p>Meeting notes can be found <a href="https://docs.google.com/document/d/1TTkAaMQV8mwUSLunZ28Ag2lu80zlN-QHa7FgEX0LMdk/edit?usp=sharing" rel="nofollow">here</a></p> <p>All the DNAs are accessible under <a href="https://biobricks.org/openmta/" rel="nofollow">openMTA</a></p>
OSF does not support the use of Internet Explorer. For optimal performance, please switch to another browser.
This website relies on cookies to help provide a better user experience. By clicking Accept or continuing to use the site, you agree. For more information, see our Privacy Policy and information on cookie use.

Start managing your projects on the OSF today.

Free and easy to use, the Open Science Framework supports the entire research lifecycle: planning, execution, reporting, archiving, and discovery.