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This project, funded by ANID-CONCYTEC CovBio, aims to develop low cost and open diagnostics for SARS-CoV-2 based on two main isothermal reactions: RT-LAMP and RPA. This repository will collect data and release up-to-date progress. #### **Preliminary protocols for enzyme purification for RT, RPA and LAMP** * **BstLF**: [Expression and purification protocol][1], [genbank seq][2] and [discussion thread at ReClone][3] * **MMLV RT**: [Expression and purification protocol][4], [genbank seq][5] y [discussion thread at ReClone][6] * **HIV RT**: * **Bst-LF DNA pol**: * **MMLV**: * **Taq Pol**: * **Pfu-Sso7d**: * **Bsu**: * **uvsX**: * **uvsY**: * **gp32**: * **LbCas12a**: #### **Preliminary results** * **RT:LAMP**: [Buffers and reactions were prepared according to these open protocols][7] and [Code for reaction optimization][8]. * [Manuscript][9] and [data repo link][10] * [Open notes about RPA progress][13] Meeting notes can be found [here][11] All the DNAs are accessible under [openMTA][12] [1]: [2]: [3]: [4]: [5]: [6]: [7]: [8]: [9]: [10]: [11]: [12]: [13]:
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