Main content



Loading wiki pages...

Wiki Version:
**View animations of single-molecule orientation-localization microscopy using the polarized vortex PSF** @[osf](qx3he) **Movie S1** (Top left) Images of blinking Nile red molecules collected by the polarized vortex microscope, (tp right) a standard SMLM reconstruction of an amyloid fibril and nearby sphere-like oligomers, and (bottom right) SMOLM reconstructions of Nile red positions and azimuthal orientations $\phi$ within the regions denoted by white boxes. @[osf](pb4hr) **Movie S2** SMOLM reconstruction of Nile red 2D positions and 3D orientations throughout an amyloid fibrillar network. The beginning of the movie shows all localizations plotted together. The end of the movie separates (left) molecules oriented mostly parallel to the coverslip ($\theta \ge 70^\circ$) from (right) those tilted out of the coverslip plane ($\theta < 70 ^\circ$).
OSF does not support the use of Internet Explorer. For optimal performance, please switch to another browser.
This website relies on cookies to help provide a better user experience. By clicking Accept or continuing to use the site, you agree. For more information, see our Privacy Policy and information on cookie use.

Start managing your projects on the OSF today.

Free and easy to use, the Open Science Framework supports the entire research lifecycle: planning, execution, reporting, archiving, and discovery.