**View animations of single-molecule orientation-localization microscopy using the polarized vortex PSF** @[osf](qx3he) **Movie S1** (Top left) Images of blinking Nile red molecules collected by the polarized vortex microscope, (tp right) a standard SMLM reconstruction of an amyloid fibril and nearby sphere-like oligomers, and (bottom right) SMOLM reconstructions of Nile red positions and azimuthal orientations $\phi$ within the regions denoted by white boxes. @[osf](pb4hr) **Movie S2** SMOLM reconstruction of Nile red 2D positions and 3D orientations throughout an amyloid fibrillar network. The beginning of the movie shows all localizations plotted together. The end of the movie separates (left) molecules oriented mostly parallel to the coverslip ($\theta \ge 70^\circ$) from (right) those tilted out of the coverslip plane ($\theta < 70 ^\circ$).
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