**Figure 2. _BCL2_ expression in I-BET151 treated MV4;11 and K-562 cells.** MV4;11 and K-562 cells were treated with 500 nM I-BET151, or an equivalent volume of DMSO. Total RNA was isolated 6 hr after treatment and qRT-PCR was used to detect _BCL2_ and _B2M_ expression. Fold change in _BCL2_ expression normalized to _B2M_ and relative to DMSO is presented for I-BET151 treated MV4;11 and K-562 cells. Expression level of _BCL2_ in DMSO was assigned a value of 1. Means reported and error bars represent _SD_ from 3 independent biological repeats. Two-sample _t_-test comparing fold gene expression values from MV4;11 cells to K-562 cells; _t_(4) = 17.23, uncorrected _p_ = 6.66x10<sup>-5</sup>, _a priori_ Bonferroni adjusted significance threshold = .0167; (Bonferroni corrected _p_ = 0.0004). One-sample _t_-test comparing fold gene expression from K-562 cells to a constant of 1 (DMSO treated cells); _t_(2) = 3.53, uncorrected _p_ = 0.0719, _a priori_ Bonferroni adjusted significance threshold = .0167; (Bonferroni corrected _p_ = 0.216). One-sample _t_-test comparing fold gene expression from MV4;11 cells to a constant of 1 (DMSO treated cells); _t_(2) = 17.86, uncorrected _p_ = 0.003, _a priori_ Bonferroni adjusted significance threshold = .0167; (Bonferroni corrected _p_ = 0.009).
Access [full size image][1] and [R script][2] used to generate this figure. This script will call the [data][3] directly from the OSF.
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[Statistical Analysis component][4] contains R scripts that were used for the analysis.
[1]: https://osf.io/qr37y/
[2]: https://osf.io/5px8f/
[3]: https://osf.io/qrkn3/
[4]: https://osf.io/vmsxa/wiki/home/