**Project: Flow-milli**
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The following repository shares resources to implement millimeter sized droplets via co-flow microfluidic droplet generators.
![enter image description here][1]
**Figure 1:** An example of co-flow droplet generator developed in this study.
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##### **Objective**
Over the past decade, droplet microfluidics have become a powerful tool for investigations across single cell analysis, high throughput screening, tissue engineering, and drug discovery [1-3]. The objective of our project was to develop simple microfluidics to generate uniform millimeter-sized droplets. To this aim, laser-cut fabrication methods were developed. Droplet generators were carefully characterized with water-in-oil droplets. Next, droplet generators were used to create tunable Matrigel droplets for 3D cell cultures.
In this repository, we shares resources to enable the use the flow-milli system by other investigators.
Current list of resources include:
1. Bill of instruments and materials
2. Code developed for analysis
3. Sample CAD designs and masks developed for the study
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**References**
1. Teh, Shia-Yen, et al. "Droplet microfluidics." Lab on a Chip 8.2 (2008): 198-220.
2. Chen, Zhengkun, et al. "Trends in droplet microfluidics: from droplet generation to biomedical applications." Langmuir 38.20 (2022): 6233-6248.
3. Wang, Yihe, et al. "Recent methods of droplet microfluidics and their applications in spheroids and organoids." Lab on a Chip (2023).
**Acknowledgments**
##### Authors acknowledge Hui-lab.
##### This project was in collaboration between Ayala lab and [Ahrar lab][2] at CSULB department of Biomedical Engineering.
Contributors:
Cory Arnold; Gabriela Pena Carmona; David A. Quiroz; Melinda G. Simon, Perla Ayala, and Siavash Ahrar
Equal contributions by: CA; GC; and DQ;
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Last updated
SA [May.6];
SA [March.11];
SA [March.9]
[1]: https://files.osf.io/v1/resources/fcr8w/providers/osfstorage/64567f7048dc1f0840bd6e2a?mode=render
[2]: http://www.svahrarlab.com
