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**Project: Flow-milli** ----------------------- The following repository shares resources to implement millimeter sized droplets via co-flow microfluidic droplet generators. ![enter image description here][1] **Figure 1:** An example of co-flow droplet generator developed in this study. ---------- ##### **Objective** Over the past decade, droplet microfluidics have become a powerful tool for investigations across single cell analysis, high throughput screening, tissue engineering, and drug discovery [1-3]. The objective of our project was to develop simple microfluidics to generate uniform millimeter-sized droplets. To this aim, laser-cut fabrication methods were developed. Droplet generators were carefully characterized with water-in-oil droplets. Next, droplet generators were used to create tunable Matrigel droplets for 3D cell cultures. In this repository, we shares resources to enable the use the flow-milli system by other investigators. Current list of resources include: 1. Bill of instruments and materials 2. Code developed for analysis 3. Sample CAD designs and masks developed for the study ---------- **References** 1. Teh, Shia-Yen, et al. "Droplet microfluidics." Lab on a Chip 8.2 (2008): 198-220. 2. Chen, Zhengkun, et al. "Trends in droplet microfluidics: from droplet generation to biomedical applications." Langmuir 38.20 (2022): 6233-6248. 3. Wang, Yihe, et al. "Recent methods of droplet microfluidics and their applications in spheroids and organoids." Lab on a Chip (2023). **Acknowledgments** ##### Authors acknowledge Hui-lab. ##### This project was in collaboration between Ayala lab and [Ahrar lab][2] at CSULB department of Biomedical Engineering. Contributors: Cory Arnold; Gabriela Pena Carmona; David A. Quiroz; Melinda G. Simon, Perla Ayala, and Siavash Ahrar Equal contributions by: CA; GC; and DQ; ---------- Last updated SA [May.6]; SA [March.11]; SA [March.9] [1]: [2]:
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