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Description: Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

License: MIT License

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Data and analysis supporting

  1. Markin, C.J.*, Mokhtari, D.A.*, Sunden, F., Appel, M.J., Akiva, E., Longwell, S.A., Sabatti, C., Herschlag, D.‡, & Fordyce, P.M.‡, Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics. bioRxiv (2020); https://doi.org/10.1101/2020.11.24.383182

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Citation

Components

Kinetic Data: cMUP Hydrolysis


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Kinetic Data: MeP Hydrolysis


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Kinetic Data: Phosphate Inhibition


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Kinetic Data: MecMUP Hydrolysis


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Kinetic Data: Varied Expression Conditions


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Kinetic Data: Urea, Zinc, and pH Titrations


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Structural Representations

Pymol (.pse) sessions for Main Body and select SI figures

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Code

Data acquisition and analysis codebases, from Fordyce Github

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Sequences and Parameter Summary


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