The replication of Study 35: [Chen et al., 2012][1] was [not pursued][2]; however, this project contains all information pertaining to the attempted replication of key experiments from this paper. It includes the detailed protocols, including reagents and author clarifications where they are available.
**Original citation:**
Chen J., Li Y., Yu T.-S., McKay R.M., Burns D.K., Kernie S.G., Parada L.F. A restricted cell population propagates glioblastoma growth after chemotherapy. Nature. 2012 Aug 23;488(7412):522-6. doi: 10.1038/nature11287.
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Note: This study had been cut from the Reproducibility Project: Cancer Biology due to inability to obtain necessary reagents.
[1]: http://www.nature.com/nature/journal/v488/n7412/full/nature11287.html
[2]: https://osf.io/ucpye/