## Registered report: BET bromodomain inhibition as a therapeutic strategy to target c-Myc <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘BET bromodomain inhibition as a therapeutic strategy to target c-Myc’ by Delmore and colleagues, published in *Cell* in 2011 ([Delmore et al., 2011][2]). The key experiments that will be replicated are those reported in Figures 3B and 7C-E. Delmore and colleagues demonstrated that treatment with JQ1, a small molecular inhibitor targeting BET bromodomains, resulted in the transcriptional down-regulation of the c-Myc oncogene in vitro (Figure 3B; [Delmore et al., 2011][2]). To assess the therapeutic efficacy of JQ1 in vivo, mice bearing multiple myeloma (MM) lesions were treated with JQ1 before evaluation for tumor burden and overall survival. JQ1 treatment significantly reduced disease burden and increased survival time (Figure 7C-E; [Delmore et al., 2011][2]). The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][3] and [Science Exchange][4] and the results of the replications will be published in *[eLife][5]*. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 19: Delmore et al., 2011 (*Cell*). The published Registered Report can be found [here][6]. **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - [Study 19 Registered Report initial draft][7] **Data Estimation**: - When original data was unable to be obtained, or original qualitative data was quantified, the estimated data is contained here. Examples include densitometric quantification of Western blot bands, or estimations of means and variances from published graphs. - [Study 19 Data Estimation][8] **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. Note: AUC = area under the curve. - [Study 19 Power Calculations.xlxs][11] - [Study 19 control vs (+)-JQ1 80 power screen shot.png][12] - [Study 19 control vs (+)-JQ1 94 power screen shot.png][13] - [Study_19_Protocol_1_2-way_ANOVA.R][14] - [Study_19_Protocol_1_tscore_from_published_p_value.R][15] - [Study_19_Protocol_2_1-way_ANOVA.R][16] - [Study_19_Protocol_2_ANCOVA.R][17] - [Study_19_Protocol_2_AUC.R][18] [1]: https://osf.io/e81xl/wiki/home/ [2]: http://www.cell.com/cell/abstract/S0092-8674%2811%2900943-3?_returnURL=http://linkinghub.elsevier.com/retrieve/pii/S0092867411009433?showall=true [3]: https://cos.io/ [4]: https://www.scienceexchange.com/ [5]: https://elifesciences.org/ [6]: https://elifesciences.org/content/4/e07072 [7]: https://osf.io/i7smn/ [8]: https://osf.io/qudi2/ [9]: https://osf.io/aw87h/ [10]: https://osf.io/kcsf6/ [11]: https://osf.io/ydrpz/ [12]: https://osf.io/5fek8/ [13]: https://osf.io/e6mbs/ [14]: https://osf.io/mqy4w/ [15]: https://osf.io/ictqb/ [16]: https://osf.io/7u2bd/ [17]: https://osf.io/72i36/ [18]: https://osf.io/9p4em/