**Figure 4. TUNEL staining of mouse tissues.** Mice bearing orthotopic 22Rv1 human prostate tumors were intravenously injected with PBS alone (PBS), 1 mg/kg DOX and PBS (DOX + PBS), or 1 mg/kg DOX and 4 µmol/kg of iRGD (DOX + iRGD). TUNEL staining was performed on tumor and heart sections of each animal. (**A**) Boxplot of mean apoptotic index calculated from TUNEL stained tumor sections. TUNEL scores were normalized to the average score of tumors from PBS treated mice. Means reported and error bars represent s.e.m. Number of mice per condition (n=6; n=18 mice total). One-way ANOVA on apoptotic index of all groups; _F_(2, 15) = 1.378, _p_ = .282. Planned contrast between DOX + PBS and DOX + iRGD; _t_(15) = 0.435, _p_ = .670 with _a priori_ alpha level = .05. Representative images of TUNEL staining of tumor sections from PBS (**B**), DOX + PBS (**C**), or DOX + iRGD (**D**) treated mice. Representative images of TUNEL staining of heart sections from PBS (**E**), DOX + PBS (**F**), or DOX + iRGD (**G**) treated mice.
Access [full size image][1] and [svg file][2] made with [Inkscape][3], version 0.91. [Boxplot][4] was generated with this [R script][5] and will call the [data][6] directly from the OSF. Representative TUNEL images for tumor sections are: (**B**) PBS; [2-PT-TUNELx40-3.3.jpg][7], (**C**) DOX + PBS; [13-PT-TUNELx40-2.2.jpg][8], (**D**) DOX + iRGD; [20-PT-TUNELx40-7.5.jpg][9]. Representative TUNEL images for heart sections are: (**E**) PBS; [003-27-H-3A.jpg][10], (**F**) DOX + PBS; [13-H-3A.jpg][11], (**G**) DOX + iRGD; [17-H-3A.jpg][12]. All experimental TUNEL images can be found [here][13].
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**Figure 4 - figure supplement 1.** This is the same experiment as in Figure 4, but with the apoptotic index plotted for each condition instead of the apoptotic index relative to PBS treated tumors. Mice bearing orthotopic 22Rv1 human prostate tumors that were intravenously injected with PBS alone (PBS), 1 mg/kg DOX and PBS (DOX + PBS), or 1 mg/kg DOX and 4 μmol/kg of iRGD (DOX + iRGD). (**A**) Boxplot of mean apoptotic index calculated from TUNEL stained tumor sections. Means reported and error bars represent s.e.m. Number of mice per condition (n=6;, n=18 mice total). (**B**) Representative image of positive control staining. Positive controls are thymus from C57BL/6J mice injected with 10 mg/kg dexamethasone that were processed in parallel to the tumor and heart sections. (**C**) Representative image of negative control staining. Negative controls are a slice taken from each tumor and processed in parallel to the test tissue, but without the TUNEL reagent. Image shown is from a DOX + iRGD treated tumor.
Access [full size image][14] and [svg file][15] made with [Inkscape][16], version 0.91. [Boxplot][17] was generated with this [R script][18] and will call the [data][19] directly from the OSF. Representative TUNEL images for (**B**) positive control; [Thymus2-TUNELx40-Pos-29.2-G.jpg][20], (**C**) negative control; [20-PT-TUNELx40-Neg-1.3.jpg][21]. All TUNEL quality control images can be found [here][22].
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[Statistical Analyses component][23] contains R scripts that were used for the analysis.
[1]: https://osf.io/57pxe/
[2]: https://osf.io/m857f/
[3]: https://inkscape.org/en/
[4]: https://osf.io/rwxst/
[5]: https://osf.io/jbfxh/
[6]: https://osf.io/5sr6y/
[7]: https://osf.io/43rp8/
[8]: https://osf.io/z3vc9/
[9]: https://osf.io/7a6bz/
[10]: https://osf.io/h6vdf/
[11]: https://osf.io/vd5jm/
[12]: https://osf.io/wfk9u/
[13]: https://osf.io/3fs27/wiki/home/
[14]: https://osf.io/2xy9p/
[15]: https://osf.io/nrk9s/
[16]: https://inkscape.org/en/
[17]: https://osf.io/hzscu/
[18]: https://osf.io/k5enf/
[19]: https://osf.io/5sr6y/
[20]: https://osf.io/7sc4d/
[21]: https://osf.io/tv64n/
[22]: https://osf.io/gmcyt/wiki/home/
[23]: https://osf.io/xn3up/wiki/home/
