This project contains all the data and Matlab scripts (v2018a) required to reproduce the figures and statistical analysis of our paper: Crijns Els, Kaliukhovich Dzmitry A., Vankelecom Lara, Op de Beeck Hans (2019) "Unsupervised Temporal Contiguity Experience Does Not Break the Invariance of Orientation Selectivity Across Spatial Frequency" Frontiers in Systems Neuroscience Vol.13. https://doi.org/10.3389/fnsys.2019.00022 The Github repository contains the basic scripts for clustering analysis. However these are not specific to this project. Also the raw data required for this analysis is not provided due to its size. Publication abstract The images projected onto the retina can vary widely for a single object. Despite these transformations primates can quickly and reliably recognize objects. At the neural level, transformation tolerance in monkey inferotemporal cortex is affected by the temporal contiguity statistics of the visual input. Here we investigated whether temporal contiguity learning also influences the basic feature detectors in lower levels of the visual hierarchy, in particular the independent coding of orientation and spatial frequency (SF) in primary visual cortex. Eight male Long Evans rats were repeatedly exposed to a temporal transition between two gratings that changed in SF and had either the same (control SF) or a different (swap SF) orientation. Electrophysiological evidence showed that the responses of single neurons during this exposure were sensitive to the change in orientation. Nevertheless, the tolerance of orientation selectivity for changes in SF was unaffected by the temporal contiguity manipulation, as observed in 239 single neurons isolated pre-exposure and 234 post-exposure. Temporal contiguity learning did not affect orientation selectivity in V1. The basic filter mechanisms that characterize V1 processing seem unaffected by temporal contiguity manipulations.