Target of replication: *A priori* experimental protocols, materials, and confirmatory analysis plans were peer reviewed, [pre-registered][1], and published by [eLife][2]. This component includes all data, analysis scripts, and methods for the replication of the experiment reported in Figures 3A-D of [Ward et al., 2010][3]. In this experiment, HEK293T cells expressing ectopic wild-type IDH2, R172K mutant IDH2, or vector control were analyzed for accumulation of the metabolite 2HG to test if R172K mutant IDH2 acquired enhanced conversion of alpha-ketoglutarate to 2HG. <br> ### Contents [Figures component][4] contains a visualization of replication results. These figures are the same as can be found in the Replication Study. [Statistical Analyses][5] component contains R scripts that were used for the analysis. [Data component][6] contains data generated from the experimental work and files used in the analysis and generation of figures, which are in .csv format. [Methods component][7] contains additional methodology details for this replication attempt which were described in the [Registered Report][8]. [Cell line quality control data][9] includes results confirming the cell lines were free of mycoplasma contamination. [Plasma line quality control data][10] includes results from the generation of the plasmids used in this study, including sequencing data confirming the correct sequence of the insert. [1]: https://osf.io/t873r/ [2]: https://elifesciences.org/content/5/e12626 [3]: http://www.cell.com/cancer-cell/abstract/S1535-6108%2810%2900036-X [4]: https://osf.io/cj8dc/wiki/home/ [5]: https://osf.io/8nd3a/wiki/home/ [6]: https://osf.io/7gkt2/wiki/home/ [7]: https://osf.io/q6tvr/wiki/home/ [8]: https://osf.io/zt996/wiki/home/ [9]: https://osf.io/zt996/wiki/home/ [10]: https://osf.io/zqyyq/wiki/home/