Production of 2HG from wild-type or R172K mutant IDH2 transfected cells

Megan Showalter; Jason Hatakeyama; Tomas Cajka; Kacey Vandervorst; Kermit L. Carraway III; Oliver Fiehn; Elizabeth Iorns; Alexandria Denis; Nicole Perfito; Tim Errington

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Study 16: Replication of Ward et al., 2010 (Cancer Cell) /

Production of 2HG from wild-type or R172K mutant IDH2 transfected cells

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Affiliated institutions: Center For Open Science

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Target of replication: A priori experimental protocols, materials, and confirmatory analysis plans were peer reviewed, pre-registered, and published by eLife. This component includes all data, analysis scripts, and methods for the replication of the experiment reported in Figures 3A-D of Ward et al., 2010. In this experiment, HEK293T cells expressing ectopic wild-type IDH2, R172K mutant IDH2, or v...