Introduction In this study we aim to investigate on one of the most important components of working memory (WM), namely early post-stimulus storage, as this has been sparsely studied (Chapman et al., 2015). We will look at the effect of negative emotion on the event related potential (ERP) C250 and further provide evidence of it being the temporal electrophysiological biomarker for early post-stimulus storage component of WM. We hypothesise that negative emotional affect will lead to higher amplitude of the C250 and enhanced WM function compared to neutral emotional affect. Methods An ERP study will be conducted on twenty healthy young adults. Participants will take part in an approximately two-hour electroencephalography (EEG) session during which they will be asked to perform a number-letter task (Chapman et al., 2015) testing WM while negative and neutral emotional affect would be induced using images from the International affective picture system. Simultaneous ratings of perceived affect will be acquired during the experiment. Approach for statistical analysis For ERP analysis, averages of artifact-free EEG trials will be generated for conditions of negative and neutral emotional affect. Grand-average ERP waveforms will be generated. This study will focus on the C250 ERP. To investigate the differences in effects of negative and neutral emotion on C250, we will apply a mixed repeated measure analysis of variance (ANOVA) to C250 amplitude and latency data with emotional affect (Negative, Neutral), task relevancy (Relevant, Irrelevant), stimulus types (Number, Letter), 4 Intratrial parts and Scalp regions (Frontal, Central, parietal and occipital) using SPSS. Effect size would be calculated as partial •2 and statistical significance would be considered with p • 0.05. To see the correlation of C250 amplitude with rating of emotional affect, Pearson or Spearman correlation test will be performed depending on the normality of data.