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Description: Published as: Behnke, M., Buchwald, M., Bykowski, A. et al. Psychophysiology of positive and negative emotions, dataset of 1157 cases and 8 biosignals. Sci Data 9, 10 (2022). https://doi.org/10.1038/s41597-021-01117-0 Subjective experience and physiological activity are fundamental components of emotion. There is an increasing interest in the link between experiental and physiological processes across different disciplines, e.g., psychology, economics, or computer science. However, the findings largely rely on sample sizes that have been modest at best (limiting the statistical power) and capture only some concurrent biosignals (e.g., making it problematic to seek specificity of some effects). We present a novel publicly available dataset of psychophysiological responses to positive and negative emotions that offers some improvement over other databases. This database involves recording of 1157 healthy participants, collected across seven studies, a continuous recordof self-reported affect along with several biosignals (electrocardiogram, impedance cardiogram, electrodermal activity, hemodynamic measures, e.g., blood pressure), respiration trace, and skin temperature. We experimentally elicited a wide range of positive and negative emotions, including amusement, anger, disgust, excitement, fear, gratitude, sadness, tenderness, and threat. Psychophysiology of positive and negative emotions (POPANE) database is currently the largest and the most comprehensive psychophysiological dataset on elicited emotions. Publication: https://doi.org/10.1038/s41597-021-01117-0 Project website: https://data.psychosensing.psnc.pl/popane/index.html Cite as: Behnke, M., Buchwald, M., Bykowski, A. et al. Psychophysiology of positive and negative emotions, dataset of 1157 cases and 8 biosignals. Sci Data 9, 10 (2022). https://doi.org/10.1038/s41597-021-01117-0