This guide will show you through how to assemble a Time-domain single photon-excited autofluorescence lifetime detection system it is divided into the three sub-sections listed below. ## Contents 1. _[Intro](https://osf.io/7w4yd/wiki/Intro%20to%20Detection%20Techniques/)_ - Brief introduction to fluorescence lifetime detection techniques. 2. _[Peer Reviewer Response Documents ](https://osf.io/7w4yd/wiki/Responses%20to%20Reviewers/)_ - Documents Addressing Peer Reviewers Feedback 3. _[Parts List](https://osf.io/7w4yd/wiki/Parts%20List/)_ - list of parts with links and prices. 4. _[Build Guide](https://osf.io/7w4yd/wiki/Build%20Guide/)_ - detailed instructions of assembly along with photos showing our actual device. 5. _[Code](https://osf.io/7w4yd/wiki/Code/)_ - includes code used for fitting and analysing the decays.
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