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<p>Target of replication: <em>A priori</em> experimental protocols, materials, and confirmatory analysis plans were peer reviewed, <a href="https://osf.io/9hr2d/" rel="nofollow">pre-registered</a>, and published by <a href="http://elifesciences.org/content/4/e06959v1" rel="nofollow"><em>eLife</em></a>.</p> <p>This component includes all primary data, analysis scripts, and QC data for the replication of the experiment reported in Figure 2D of <a href="http://science.sciencemag.org/content/328/5981/1031" rel="nofollow">Sugahara et al. 2010</a>. This experiment was performed to assess the impact on cellular apoptosis (as defined by TUNEL) of tumors and heart in mice that were treated with 1 mg/kg DOX + PBS, or 1 mg/kg DOX + 4 ┬Ámol/kg for 24 days.</p> <p><br></p> <h3>Contents</h3> <p><a href="https://osf.io/bfgd3/wiki/home/" rel="nofollow">Figures component</a> contains a visualization of replication results. These figures are the same as can be found in the Replication Study and include Figure 4 displaying TUNEL scoring in mouse tissue.</p> <p><a href="https://osf.io/xn3up/wiki/home/" rel="nofollow">Statistical Analyses component</a> contains R scripts that were used for the analyses.</p> <p><a href="https://osf.io/3fs27/wiki/home/" rel="nofollow">Data Component</a> contains data generated from the experimental work and files used in the analysis and generation of figures, which are in .csv format.</p> <p><a href="https://osf.io/gmcyt/wiki/home" rel="nofollow">TUNEL control data component</a> contains the negative and positive controls sections that were stained in parallel to the tumor and heart samples.</p> <p><a href="https://osf.io/bkhnp/wiki/home" rel="nofollow">Methods component</a> contains methodology details for this replication attempt which were described in the <a href="http://elifesciences.org/content/4/e06959v1" rel="nofollow">Registered Report</a>.</p> <p><a href="https://osf.io/en6ru/wiki/home" rel="nofollow">Cell line quality control data</a> includes results confirming the cell lines were free of mycoplasma contamination and common mouse pathogens as well as STR DNA profiling of the cell lines used.</p> <p><a href="https://osf.io/p73gy/wiki/home/" rel="nofollow">iRGD peptide component</a> contains the methods and quality control data generated during the synthesis.</p>
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