Supplementary Figure 1 (A) Representative H&E staining of GARD phenotype in kidney of a C57BL/6JRj mouse with the Dhtkd1335T/335T genotype. (B) Urinary protein in 20-months old C57BL/6J (Dhtkd1335T/335T) mice aged in our own facility compared to historic samples of C57BL/6J (Dhtkd1335T/335T) mice with a GARD phenotype. (C) Representative H&E staining of kidney section and (D) transmission electron microscopy picture of a glomerulus from a 24-month old C57BL/6J (Dhtkd1335T/335T) mouse showing no GARD. Data are mean ± SEM, (n=9-12). Significant differences are indicated with **p<0.001.
Supplementary Figure 2 (A) Representative immunofluorescence for DHTKD1 and mitochondrial marker MTC02 in DHTKD1-overexpressing HK2 cells. F-actin was stained using labeled Phalloidin. (B) Representative immunofluorescence for DHTKD1 and phalloidin-labeling of F-actin in DHTKD1-overexpressing human podocytes.
