To facilitate locating the materials for each experiment, we compiled this list as a reference index for all materials related to the individual replication experiments. Links direct to: **Registered protocols:** Time-stamped version of the protocol registered prior to experiments were conducted. **Post hoc protocol amendments:** Notes that list all adaptations made during the execution of each experiment. **Full datasets:** Data collection spreadsheets filled in by each replication laboratory, including all data collected during execution of the experiment. **Processed datasets:** Standardised tables for individual-level data for the primary outcomes obtained from the full datasets. Secondary outcomes are available for EPM experiments [here][1]. Unless specified as 'LABUNIT', all tables include the experimental unit considered correct by the coordinating team. **Scatter plots:** Unit-level scatter plots for primary outcomes, created from the processed datasets. Unless specified as 'no outliers', all experimental units considered valid are shown in each plot. ---------- Elevated Plus Maze (EPM) experiments ------------------------------------ **EPM01** *Swiss albino mice were divided into a cafeteria diet group, which provides 4.12 kcal/g, and a control group (2.93 kcal/g). Both groups were kept on their respective diet for 13 weeks and submitted to behavioral tests. The primary comparison of interest in the replication is between time spent in open arms by mice chronically exposed or not to the cafeteria diet.* Registered protocols: [LAB16][2], [LAB80][3], [LAB82][4] Post hoc protocol amendments: [LAB16][5], [LAB80][6], [LAB82][7] Full datasets: [LAB16][8], [LAB80][9], [LAB82][10] Processed datasets: [LAB16][11], [LAB80][12], [LAB82][13] Scatter plots: [LAB16][14], [LAB80][15], [LAB82][16] **EPM02** *Adult male Swiss mice received intraperitoneal injections of the benzodiazepine chlordiazepoxide (5 mg/kg) or saline (control). 30 min later, they were exposed to the EPM. The comparison of interest in the replication is between the percentage of time in open arms by mice treated with chlordiazepoxide and those treated with saline.* Registered protocols: [LAB07][17], [LAB24][18], [LAB86][19] Post hoc protocol amendments: [LAB07][20], [LAB24][21], [LAB86][22] Full datasets: [LAB07][23], [LAB24][24], [LAB86][25] Processed datasets: [LAB07][26], [LAB24][27], [LAB86][28] Scatter plots: [LAB07][29], [LAB24][30], [LAB86][31] **EPM05** *Male Wistar rats were submitted daily to 14 h of water deprivation, followed by 10 h of water ad libitum during 18 treatment days. Every day, after water deprivation, a diazepam solution(diazepam10 mg/mL+ 5% propylene glycol + 5% sucrose) was offered to the experimental group for 30 min during the first 2 days and for 10 min in the remaining ones. For the control group, a 5% propylene glycol + 5% sucrose solution, without diazepam, was offered in the same schedule. 48 h after the last ingestion, animals were submitted to EPM. The comparison of interest in the replication is between the percentage of time spent in open arms by rats treated with diazepam and those treated with the sucrose solution only.* Registered protocols: [LAB07][32], [LAB10][33], [LAB62][34] Post hoc protocol amendments: [LAB07][35], [LAB10][36], [LAB62][37] Full datasets: [LAB07][38], [LAB10][39], [LAB62][40] Processed datasets: [LAB07][41], [LAB10][42], [LAB62][43] Scatter plots: [LAB07][44], [LAB10][45], [LAB62][46] **EPM09** *Male Swiss mice were treated orally with 400 mg/kg of the monoterpene 1,4-cineole emulsified in 0.2% Tween dissolved in distilled water. Control animals received only vehicle (saline with 0.2% Tween). One hour after treatment, the EPM task was performed. The comparison of interest in the replication is between the time spent in open arms by mice treated with 1,4-cineole (400 mg/kg) and those treated with vehicle.* Registered protocols: [LAB07][47], [LAB24][48], [LAB80][49] Post hoc protocol amendments: [LAB07][50], [LAB24][51], [LAB80][52] Full datasets: [LAB07][53], [LAB24][54], [LAB80][55] Processed datasets: [LAB07][56], [LAB24][57], [LAB80][58] Scatter plots: [LAB07][59], [LAB24][60], [LAB80][61] **EPM11** *Male Wistar rats aged 13-15 weeks received intraperitoneal injections of the non-competitive NMDA antagonist memantine (8 mg/kg) or saline. 30 minutes later they were exposed to the EPM. The comparison of interest in the replication is between the percentage of time spent in the open arms by the rats treated with memantine and those treated with saline.* Registered protocols: [LAB62][62], [LAB80][63], [LAB83][64] Post hoc protocol amendments: [LAB62][65], [LAB80][66], [LAB83][67] Full datasets: [LAB62][68], [LAB80][69], [LAB83][70] Processed datasets: [LAB62][71], [LAB80][72], [LAB83][73] Scatter plots: [LAB62][74], [LAB80][75], [LAB83][76] **EPM13** *From day 15 to 21 of pregnancy, female Wistar rats in the experimental group were stressed by 30-min restraint sessions in a Plexiglas apparatus (24 x 9 x 5 cm) 4 times a day, while control rats were not. After birth, pups were cross-fostered to different mothers, either in the same or in the other group. The comparison of interest in the replication is between the percentage of time spent in enclosed arms in the EPM by the adult offspring that were both born from and reared by non-stressed dams (NS:NS) and the ones both born from and reared by stressed dams (S:S).* Registered protocols: [LAB11][77], [LAB38][78], [LAB87][79], [LAB91][80] Post hoc protocol amendments: [LAB11][81], [LAB91][82] Full datasets: [LAB11][83], [LAB91][84] Processed datasets: [LAB11][85], [LAB91][86] Scatter plots: [LAB11][87], [LAB91][88] **EPM15** *Adult female Wistar rats had free access to water or caffeine (1 g/L) in alternated periods during the light-dark cycle for 2 weeks, 5 days/week during mating and pregnancy. After giving birth, they were kept on either caffeine or water during the lactation period. After weaning, male pups either kept receiving caffeine in the same protocol (5 days/week, dark cycle only) or received tap water for 10 weeks. When they reached P90, male rats were exposed to an open field test and a novel object recognition task on two consecutive days. EPM task was performed on the third day. The comparison of interest in the replication is between the time spent in open arms by rats treated with caffeine (1 g/L) from development to adulthood and those treated with water during the same period.* Registered protocols: [LAB11][89], [LAB24][90] Post hoc protocol amendments: [LAB11][91] Full datasets: [LAB11][92] Processed datasets: [LAB11][93] Scatter plots: [LAB11][94] **EPM18** *Pregnant Wistar rats were assigned to receive tap water or ethanol (22.5%, 6.5 g/kg) by gavage for 21 days of gestation and 21 days of breastfeeding. When offspring were 2 months old, the open field and elevated plus maze tests were conducted on both male and female animals. The comparison of interest in the replication is between the percentage of open arm entries by the rats that were pre- and perinatally exposed to ethanol and those that were not.* Registered protocols: [LAB16][95], [LAB38][96] Post hoc protocol amendments: -- Full datasets: -- Processed datasets: -- Scatter plots: -- **EPM19** *Adult male Swiss mice were exposed to the monoterpene linalool oxide by inhalation for 7 min in an acrylic chamber with holes, in which cotton balls embedded in a solution of linalool oxide 0.65% were inserted. Control animals were exposed to the same chamber, but with cotton balls embedded in vehicle (Tween 80 0.2%). Shortly after this exposure, animals were subjected to the EPM. The comparison of interest in the replication is between the number of open arm entries by mice exposed to linalool oxide and those that were not.* Registered protocols: [LAB07][97], [LAB24][98] Post hoc protocol amendments: [LAB07][99], [LAB24][100] Full datasets: [LAB07][101], [LAB24][102] Processed datasets: [LAB07][103], [LAB24][104] Scatter plots: [LAB07][105], [LAB24][106] **EPM21** *Male Swiss mice were weaned at 21 days old and housed in pairs. After 14 days, these pairs were separated in two groups. In the experimental group, one of the animals in the pair was restrained in a 14 x 3 cm pipe for 1 h daily for 14 days to induce stress, while its cage-mate observed. On the same experimental days, the control pairs were moved to an adjacent room, but no stress was induced in any mouse. After this period of chronic stress, the elevated plus maze test was performed. The comparison of interest in the replication is between the percentage of open arm entries by the cagemates of stressed animals and animals from the control group.* Registered protocols: [LAB24][107], [LAB80][108] Post hoc protocol amendments: [LAB24][109], [LAB80][110] Full datasets: [LAB24][111], [LAB80][112] Processed datasets: [LAB24][113], [LAB80][114] Scatter plots: [LAB24][115], [LAB80][116] **EPM25** *Adult male Wistar rats received methylscopolamine (1 mg/kg) subcutaneously, followed 30 min later by an intraperitoneal injection of saline (control) or the cholinergic agonist pilocarpine in a subconvulsant dose (75 mg/kg). Animals were evaluated in the EPM 24 h later. The comparison of interest in the replication is between the percentage of time spent in open arms by rats treated with methylscopolamine and pilocarpine (75 mg/kg) and those that were treated with methylscopolamine and saline.* Registered protocols: [LAB25][117], [LAB62][118], [LAB83][119] Post hoc protocol amendments: [LAB25][120], [LAB62][121], [LAB83][122] Full datasets: [LAB25][123], [LAB62][124], [LAB83][125] Processed datasets: [LAB25][126], [LAB62][127], [LAB83][128] Scatter plots: [LAB25][129], [LAB62][130], [LAB83][131] **EPM27** *Wistar rats born from different dams were pooled together and assigned to be reared in unfavorable conditions – i.e large litters of 15 pups/dam. Male pups received L-glutamine (500 mg/kg) or distilled water by gavage daily from p7 to p27. At p28, male animals were exposed to the EPM. The comparison of interest in the replication is between the total distance traveled in the maze between rats reared under unfavorable conditions that received L-glutamine (500 mg/kg) supplementation and those that were reared under the same conditions but received water.* Registered protocols: [LAB16][132], [LAB38][133], [LAB87][134] Post hoc protocol amendments: [LAB16][135], [LAB87][136] Full datasets: [LAB16][137], [LAB87][138] Processed datasets: [LAB16][139], [LAB87][140] Scatter plots: [LAB16][141], [LAB87][142] **EPM28** *Female Wistar rats, weighing 150-200 g, were treated with venlafaxine (10 mg/kg) or saline administered orally daily for 10 days. After 8 days of treatment, all animals underwent a protocol for REM sleep deprivation, where they stood on a small platform in a tank of water for 72 hours, so as to be woken up when falling into REM sleep. After the sleep deprivation period, the EPM procedure was performed. The comparison of interest in the replication is between time spent in open arms by sleep-deprived rats treated with venlafaxine (10 mg/kg) and those that were sleep-deprived but treated with saline.* Registered protocols: [LAB07][143], [LAB82][144], [LAB85][145] Post hoc protocol amendments: [LAB07][146], [LAB82][147], [LAB85][148] Full datasets: [LAB07][149], [LAB82][150], [LAB85][151] Processed datasets: [LAB07][152], [LAB82][153], [LAB85][154] Scatter plots: [LAB07][155], [LAB82][156], [LAB85][157] **EPM29** *12-week-old male Wistar rats were injected subcutaneously with diphenyl ditelluride (3.1 μg/kg), an organic tellurium compound, 5 days a week up until the accumulated dose of 124 μg/kg (i.e. 8 weeks). Controls received only vehicle (canola oil) on the same days. 72 h after the last injection, the EPM task was performed. The comparison of interest in the replication is between time spent in open arms by rats chronically exposed to diphenyl ditelluride and those exposed to vehicle.* Registered protocols: [LAB10][158], [LAB85][159], [LAB88][160] Post hoc protocol amendments: [LAB10][161], [LAB85][162], [LAB88][163] Full datasets: [LAB10][164], [LAB85][165], [LAB88][166] Processed datasets: [LAB10][167], [LAB85][168], [LAB88][169] Scatter plots: [LAB10][170], [LAB85][171], [LAB88][172] **EPM31** *Adult male Wistar rats went through stereotaxic surgery to implant a single canula at the dorsomedial hypothalamic region. 7 days later, animals received an intra-cerebral injection of the benzodiazepine midazolam maleate (60 nmol) or saline. 10 min after the injection, animals were exposed to the EPM. The comparison of interest in the replication is between the percentage of entries in open arms by rats treated with midazolam (60 nmol) and those treated with saline.* Registered protocols: [LAB10][173], [LAB76][174], [LAB86][175] Post hoc protocol amendments: [LAB10][176], [LAB76][177], [LAB86][178] Full datasets: [LAB10][179], [LAB76][180], [LAB86][181] Processed datasets: [LAB10][182], [LAB76][183], [LAB86][184] Scatter plots: [LAB10][185], [LAB76][186] ([no outliers][187]), [LAB86][188] **EPM37** *Male Wistar rats were implanted with a unilateral guide-cannula into the right central nucleus of the amygdala. After recovery from surgery, 0.2 μL of saline (control) or the GABAergic agonist muscimol (1 nmol) were injected through the cannula and animals were tested in the EPM 15 min later. The comparison of interest in the replication is between the number of entries in the open arms by rats treated with 1 nmol of muscimol and saline-treated rats.* Registered protocols: [LAB10][189], [LAB76][190], [LAB82][191] Post hoc protocol amendments: [LAB10][192], [LAB76][193], [LAB82][194] Full datasets: LAB10 ([part 1][195], [part 2][196]), [LAB76][197], [LAB82][198] Processed datasets: [LAB10][199], [LAB76][200], [LAB82][201] Scatter plots: [LAB10][202], [LAB82][203] **EPM39** *Male Wistar rats, 15 days old, were divided into two groups. The experimental group received daily intraperitoneal injections of methylphenidate (2 mg/kg) for 30 days in a row. Controls received equivalent injections of vehicle (saline). 24 hours after the last injection, the EPM procedure was performed. The comparison of interest in the replication is between total number of arm entries by rats chronically treated with methylphenidate and those treated with saline.* Registered protocols: [LAB07][204], [LAB25][205] Post hoc protocol amendments: -- Full datasets: -- Processed datasets: -- Scatter plots: -- **EPM43** *Adult male Wistar rats went through stereotaxic surgery to implant a single angled canula aimed at the dorsal raphe nucleus. 6 days later, the experimental group received a 0.3-μL injection of either 100 nmol L-arginine or saline through the cannula. 10 min after the injection, animals were exposed to the EPM. The comparison of interest in the replication is between the percentage of time spent in open arms by rats treated with L-arginine (100 nmol) and those treated with saline.* Registered protocols: [LAB76][206], [LAB82][207] Post hoc protocol amendments: [LAB82][208] Full datasets: [LAB82][209] Processed datasets: [LAB82][210] Scatter plots: [LAB82][211] **EPM45** *9-week-old male Lewis rats were treated by gavage with 0.3 mg/kg of the NK1-receptor antagonist NKP608 suspended in 0.5% methylcellulose or vehicle. EPM was performed 90 minutes after treatment. The comparison of interest in the replication is between time spent in open arms by rats treated with NKP608 0.3 mg/kg and those treated with vehicle only.* Registered protocols: [LAB80][212] Post hoc protocol amendments: -- Full datasets: -- Processed datasets: -- Scatter plots: -- **EPM46** *Female Wistar rats were implanted with bilateral cannulae into the shell of the nucleus accumbens. After at least 7 days of recovery, animals received a bilateral microinjection (0.4 μL) of the AMPA antagonist DNQX (330 ng) or its vehicle (50% DMSO). Following drug administration, each animal underwent the EPM task. The comparison of interest in the replication is between the percentage of open arm entries by the rats treated with DNQX (330 ng) and those treated with DMSO.* Registered protocols: [LAB38][213], [LAB76][214], [LAB86][215] Post hoc protocol amendments: [LAB86][216] Full datasets: [LAB86][217] Processed datasets: [LAB86][218] Scatter plots: [LAB86][219] MTT assay experiments --------------------- **MTT04** *Murine RAW 264.7 macrophages were cultured in DMEM and then treated or not with a mixture of caffeine, theobromine and catechin, each at 25 μg/mL (N = 3). After 72h, cell proliferation wasg/mL (N = 3). After 72h, cell proliferation was determined by performing the MTT assay. The comparison was between cultures treated with the mixture (25 μg/mL (N = 3). After 72h, cell proliferation wasg/mL of each substance) and those that were not treated.* Registered protocols: [LAB44][220], [LAB64][221], [LAB68][222], [LAB72][223] Post hoc protocol amendments: [LAB68][224], [LAB72][225] Full datasets: [LAB68][226], [LAB72][227] Processed datasets: [LAB68][228], [LAB72][229] Scatter plots: [LAB68][230], [LAB72][231] **MTT13** *LLC-PK1 porcine proximal tubular cell lines were cultured in RPMI-1640 with 10% FBS and then treated or not with cyclosporine 10 μM diluted in medium for 1 hour. After that, cell viability wasM diluted in medium for 1 hour. After that, cell viability was determined by performing the MTT assay. The comparison was between cultures treated with cyclosporine 10 μM, 1 h) and those that were not.* Registered protocols: [LAB52][232], [LAB54][233], [LAB72][234] Post hoc protocol amendments: [LAB52][235], [LAB54][236], [LAB72][237] Full datasets: LAB52 ([LINK1][238], [LINK2][239]), [LAB54][240], [LAB72][241] Processed datasets: [LAB52][242], [LAB54][243], [LAB72][244] Scatter plots: [LAB52][245], [LAB54][246], [LAB72][247] **MTT18** *LLC-PK1 porcine proximal tubular cell lines were cultured in RPMI-1640 with 10% FBS and then treated or not with cyclosporine 10 μM diluted in medium for 1 hour. After that, cell viability wasM diluted in medium for 1 hour. After that, cell viability was determined by performing the MTT assay. The comparison was between cultures treated with cyclosporine 10 μM, 1 h) and those that were not.* Registered protocols: [LAB01][248], [LAB18][249], [LAB33][250] Post hoc protocol amendments: [LAB01][251], [LAB18][252], [LAB33][253] Full datasets: [LAB01][254], [LAB18][255], [LAB33][256] Processed datasets: [LAB01][257], [LAB18][258], [LAB33][259] Scatter plots: [LAB01][260], [LAB18][261], [LAB33][262] **MTT26** *Peritoneal macrophages were isolated from a pool of male 8-week-old Swiss mice by suction. Cells were resuspended and cultured for 24h, then treated with 300 ng/mL of an organochlorine pesticide mixture for 24h (N = 14), after which the MTT assay was performed. The comparison was between the cell cultures exposed to pesticides and unexposed cultures.* Registered protocols: [LAB50][263], [LAB55][264], [LAB84][265] Post hoc protocol amendments: [LAB55][266], [LAB84][267] Full datasets: [LAB55][268], [LAB84][269] Processed datasets: [LAB55][270], [LAB84][271] ([LABUNIT][272]) Scatter plots: [LAB55][273] ([no outliers][274]) **MTT28** *DU 145 prostate cancer cells were cultured in RPMI-1640 + 10% FBS. 24 h after plating, the culture medium was replaced so that experimental groups were treated or not with the phytosterol γ-oryzanol at 16 μM. 48h later, MTT assay procedures started, comparing cell viability between cells treated with γ-oryzanol (16 μM) and those that were not.* Registered protocols: [LAB31][275], [LAB57][276], [LAB60][277], [LAB72][278] Post hoc protocol amendments: [LAB31][279], [LAB60][280] Full datasets: [LAB31][281], [LAB60][282] Processed datasets: [LAB31][283], [LAB60][284] Scatter plots: [LAB31][285], [LAB60][286] **MTT30** *B16F10 cells were grown for 24h, then treated with monastrol (80 uM) or ethanol for 48h (N = 5). The MTT assay was then performed to compare the cell viability of the two groups.* Registered protocols: [LAB32][287], [LAB53][288], [LAB64][289], [LAB72][290] Post hoc protocol amendments: [LAB32][291], [LAB72][292] Full datasets: [LAB32][293], [LAB72][294] Processed datasets: [LAB32][295], [LAB72][296] Scatter plots: [LAB32][297], [LAB72][298] **MTT33** *LLC-MK2 proximal tubular epithelial cells were grown in DMEM + 10% FBS. They went through 24h of ischemia in an anaerobic chamber with a medium deprived of serum, glucose and pyruvate, then 3h of reperfusion in complete culture medium. After this ischemia/reperfusion (I/R) procedure, cells were treated or not with the sesquiterpene alcohol α-bisabolol, 31.25 μM, for 24h. Afterwards, the MTT assay was performed, comparing cell cultures treated with α-bisabolol to those that were not.* Registered protocols: [LAB21][299], [LAB52][300], [LAB53][301] Post hoc protocol amendments: [LAB21][302], [LAB52][303] Full datasets: [LAB21][304], LAB52 ([LINK1][305], [LINK2][306]) Processed datasets: [LAB21][307], [LAB52][308] Scatter plots: [LAB21][309], [LAB52][310] **MTT37** *PC12 rat pheochromocytoma cells were cultured in DMEM high glucose + 10% horse serum and 5% FBS. They were exposed to 1mM MPP+ iodide for 24h to induce toxicity, in the presence or absence of cannabidiol (CBD), 1 μM, for 24h. The MTT assay was performed afterward, comparing the viability of the cells treated with MPP+ (1mM) and CBD (1 μM) to those treated with MPP+ alone.* Registered protocols: [LAB21][311], [LAB22][312], [LAB28][313], [LAB33][314] Post hoc protocol amendments: [LAB21][315], [LAB28][316], [LAB33][317] Full datasets: [LAB21][318], [LAB28][319], [LAB33][320] Processed datasets: [LAB21][321], [LAB28][322], [LAB33][323] Scatter plots: [LAB21][324], [LAB28][325], [LAB33][326] **MTT42** *SV40 transformed mouse embryonic fibroblasts (MEF) were grown for 24h then treated with 20 mM of sodium salicylate (NaSal) for 16h. MTT was performed immediately after treatment. Cell viability was compared between cultures that were treated with NaSal (20 mM) and the ones that were not treated.* Registered protocols: [LAB01][327], [LAB13][328], [LAB18][329] Post hoc protocol amendments: [LAB01][330], [LAB13][331], [LAB18][332] Full datasets: [LAB01][333], [LAB13][334], [LAB18][335] Processed datasets: [LAB01][336], [LAB13][337], [LAB18][338] Scatter plots: [LAB01][339], [LAB13][340], [LAB18][341] **MTT45** *SH-SY5Y - human dopaminergic neuroblastoma cell line were grown until confluence in DMEM/F-12 HAM with 10% FBS and 2mM L-glutamine. After that, they were preincubated or not with the flavonoid pinocembrin, 25 μM, for 4h. This was then followed by incubation of both groups for 24h with the herbicide paraquat (100 μM). The MTT assay was then performed. The comparison was made between paraquat-treated cells that were pre-incubated with pinocembrin (25 μM) to those that were treated with paraquat in the absence of pre-incubation.* Registered protocols: [LAB33][342], [LAB42][343], [LAB44][344] Post hoc protocol amendments: [LAB33][345], [LAB42][346] Full datasets: [LAB33][347], [LAB42][348] Processed datasets: [LAB33][349], [LAB42][350] Scatter plots: [LAB33][351], [LAB42][352] **MTT52** *To compare the ability of Lucena-1 and K562 in reducing MTT, both cells were grown (N = 6) and the MTT assay was performed comparing the two cell lines* Registered protocols: [LAB44][353], [LAB67][354], [LAB68][355] Post hoc protocol amendments: [LAB67][356], [LAB68][357] Full datasets: [LAB67][358], [LAB68][359] Processed datasets: [LAB67][360], [LAB68][361] Scatter plots: [LAB67][362], [LAB68][363] **MTT56** *Human breast adenocarcinoma MCF-7 cells were grown in DMEM + 10% FBS and treated or not with the soy isoflavone genistein, 1 μM, for 72 hours. After treatment, the MTT assay was performed. The comparison was between cells treated with genistein (1 μM) and those that were not (control).* Registered protocols: [LAB43][364], [LAB49][365], [LAB57][366] Post hoc protocol amendments: [LAB43][367], [LAB49][368], [LAB57][369] Full datasets: [LAB43][370], [LAB49][371], [LAB57][372] Processed datasets: [LAB43][373], [LAB49][374], [LAB57][375] Scatter plots: [LAB43][376], [LAB49][377], [LAB57][378] **MTT60** *PC3 human prostate adenocarcinoma cells were grown in RPMI + 10% FBS. Cells were transfected with either a silencing RNA for the ARHGAP21 gene, encoding a member of the RhoGAP family of proteins, or a control siRNA. 24h after transfections, cells were plated and the MTT assay was performed, comparing cell viability between the cells transfected with the two RNAs.* Registered protocols: [LAB22][379], [LAB53][380], [LAB57][381] Post hoc protocol amendments: [LAB22][382], [LAB57][383] Full datasets: [LAB22][384], [LAB57][385] Processed datasets: [LAB22][386], [LAB57][387] Scatter plots: [LAB22][388], [LAB57][389] **MTT67** *The J774 mouse macrophage cell line was grown until confluence in RPMI-1640 + 10% FBS. Experimental cultures were treated with nimesulide, 10-5 M, or left with medium only. After 30 minutes, both groups were treated for 8h with LPS, 1μg/mL. After that, the MTT assay was performed, comparing the cultures that were treated with nimesulide with the ones that were not treated.* Registered protocols: [LAB31][390], [LAB33][391], [LAB47][392] Post hoc protocol amendments: [LAB31][393], [LAB33][394] Full datasets: [LAB31][395], [LAB33][396] Processed datasets: [LAB31][397], [LAB33][398] Scatter plots: [LAB31][399], [LAB33][400] ([no outliers][401]) **MTT71** *HaCat human keratinocyte cells were grown in DMEM + 10% FBS, then incubated with either the pentacyclic triterpenoid betulinic acid, 20 μM, or its vehicle, 0.25% DMSO, for 24h. After that, cells were washed, supplemented with fresh medium, and the MTT assay was performed to assess cell viability, comparing cultures incubated with betulinic acid (20 μM) to those incubated with DMSO.* Registered protocols: [LAB49][402], [LAB54][403], [LAB60][404] Post hoc protocol amendments: [LAB49][405], [LAB54][406], [LAB60][407] Full datasets: [LAB49][408], [LAB54][409], [LAB60][410] Processed datasets: [LAB49][411], [LAB54][412], [LAB60][413] Scatter plots: [LAB49][414], [LAB54][415], [LAB60][416] **MTT81** *Peritoneal macrophages from Swiss mice were maintained in culture. The cultures were incubated with or without (+)-α-pinene, 0.25 mg/mL, for 24h (N = 3), after which the MTT assay was performed, comparing the two groups.* Registered protocols: [LAB50][417], [LAB55][418], [LAB84][419], [LAB85][420] Post hoc protocol amendments: [LAB55][421], [LAB84][422], [LAB85][423] Full datasets: [LAB55][424], [LAB84][425], [LAB85][426] Processed datasets: [LAB55][427], [LAB84][428] ([LABUNIT][429]), [LAB85][430] Scatter plots: [LAB55][431], [LAB85][432] **MTT83** *MCF-7 cells were cultured and incubated with resveratrol, 1 μM, or DMSO (vehicle) for 24h (N = 7). The MTT assay was performed afterwards comparing the cultures exposed and unexposed to resveratrol.* Registered protocols: [LAB19][433], [LAB32][434], [LAB75][435] Post hoc protocol amendments: [LAB19][436], [LAB32][437], [LAB75][438] Full datasets: [LAB19][439], [LAB32][440], [LAB75][441] Processed datasets: [LAB19][442], [LAB32][443], [LAB75][444] Scatter plots: [LAB19][445], [LAB32][446], [LAB75][447] **MTT86** *KYSE30 cells were maintained in RPMI medium with 10% FBS. They were incubated for 24h with or without 5mM ATP. Then MTT assay was performed, comparing the conditions with and without ATP.* Registered protocols: [LAB18][448], [LAB19][449], [LAB42][450] Post hoc protocol amendments: [LAB18][451], [LAB19][452], [LAB42][453] Full datasets: [LAB18][454], [LAB19][455], [LAB42][456] Processed datasets: [LAB18][457], [LAB19][458], [LAB42][459] Scatter plots: [LAB18][460], [LAB19][461], [LAB42][462] **MTT87** *SCC9 human oral squamous cell carcinoma cells were cultured until 20% confluence was reached. Then they were cultured for 24h in serum-reduced medium. The experimental group was then treated for 6h with norepinephrine 10 μM, while controls remained untreated (N = 8). Then MTT assay was performed comparing the treated and untreated groups.* Registered protocols: [LAB13][463], [LAB21][464], [LAB28][465] Post hoc protocol amendments: [LAB13][466], [LAB21][467], [LAB28][468] Full datasets: [LAB13][469], [LAB21][470], [LAB28][471] Processed datasets: [LAB13][472], [LAB21][473], [LAB28][474] Scatter plots: [LAB13][475], [LAB21][476], [LAB28][477] **MTT96** *Macrophages from Swiss mice were extracted by lavage of the peritoneal cavity. Cells were plated and after 1h treated with 40 ug/mL chlorhexidine for 1h (N = 3). MTT was then performed. Comparisons were made between cultures treated with or without chlorhexidine.* Registered protocols: [LAB50][478], [LAB55][479], [LAB84][480], [LAB85][481] Post hoc protocol amendments: [LAB55][482], [LAB84][483] Full datasets: [LAB55][484], [LAB84][485], [LAB85][486] Processed datasets: [LAB55][487], [LAB84][488] ([LABUNIT][489]) Scatter plots: [LAB55][490] RT-PCR experiments ------------------ **PCR11** *The fibroblasts cultures of the 3T3-L1 cell lineage were differentiated on mature adipocytes by the cultivation on medium containing 10 μg/mL insulin, 1 μM dexamethasone, and 0.5 mM 3- isobutyl-1-methylxanthine. After that, cultures in the experimental group were incubated with 17- beta estradiol for 24h. RT-qPCR analysis was then performed using SYBR Green. Slc2a4 expression relative to GAPDH was compared between 17β-estradiol and untreated cultures. The primary outcome of this replication will be the comparison of relative expression levels of Slc2a4 and GAPDH between cells with adipose morphology treated with 17β-estradiol and non-treated cells (n = 6/ group).* Registered protocols: [LAB48][491], [LAB55][492], [LAB90][493] Post hoc protocol amendments: [LAB48][494], [LAB55][495], [LAB90][496] Full datasets: [LAB48][497], [LAB55][498], [LAB90][499] Processed datasets: [LAB48][500], [LAB55][501], [LAB90][502] Scatter plots: [LAB48][503] ([no outliers][504]), [LAB90][505] ([no outliers][506]) **PCR114** *RKO cells, a p53 wild-type human colon carcinoma cell line, were maintained in DMEM. Cells were treated with 1,4-diamino-2-butanone (DAB) at 0.3mM or not (controls) for 24 h. After that, total RNA was extracted using the Trizol kit and used to perform real-time PCR (RT-qPCR) analysis using HOT FIREPol® EvaGreen® qPCR Mix Plus. Nrf-2 expression relative to GAPDH was compared between vehicle and DAB-treated cells (n = 3/ group). The primary outcome of this replication will be the comparison of Nrf-2 mRNA levels related to GPADH between 0.3mM DAB treated and non-treated (controls) RKO cells.* Registered protocols: [LAB14][507], [LAB36][508], [LAB43][509] Post hoc protocol amendments: [LAB14][510], [LAB36][511], [LAB43][512] Full datasets: [LAB14][513], [LAB36][514], [LAB43][515] Processed datasets: [LAB14][516], [LAB36][517], [LAB43][518] Scatter plots: [LAB14][519], [LAB36][520], [LAB43][521] **PCR125** *Eight-week-old Wistar rats received one intraperitoneal injection of lead (II) acetate (25 mg/kg) or vehicle. After 24 h, animals were euthanized by exsanguination through cardiac puncture prior to decapitation and the kidneys were dissected for RNA extraction and real-time RT-PCR using GoTaq® qPCR. The comparison of interest in the replication is between TrxR1 expression relative to GAPDH and Rpl13a in vehicle-treated animals as controls and acetate-treated animals as experimental group for 24h (n = 24/ group).* Registered protocols: [LAB09][522], [LAB35][523], [LAB78][524] Post hoc protocol amendments: [LAB09][525], [LAB35][526], [LAB78][527] Full datasets: [LAB09][528], [LAB35][529], [LAB78][530] Processed datasets: [LAB09][531], [LAB35][532], [LAB78][533] Scatter plots: [LAB09][534], [LAB35][535], [LAB78][536] ([no outliers][537]) **PCR126** *SaOs-2 osteoblastic cells were maintained in DMEM + 10% FBS. They were plated and 7 days later 10 mM beta-glycerophosphate (βG) and 0.25 mM ascorbic acid (AA) were added to wells to induce calcification. After this, ascorbic acid was added every day, while beta-glycerophosphate was added every 3 days with medium changes. After 7 days, RNA was isolated and real-time PCR using TaqMan probes was performed. The primary outcome of this replication will be SLC20A2 mRNA levels expression relative to GAPDH compared between untreated control cells and AA+βG treated cells after 7 days (n = 4/ group).* Registered protocols: [LAB09][538], [LAB22][539], [LAB27][540] Post hoc protocol amendments: [LAB22][541], [LAB27][542] Full datasets: [LAB22][543], [LAB27][544] Processed datasets: [LAB22][545], [LAB27][546] ([LABUNIT][547]) Scatter plots: [LAB22][548] **PCR139** *K562 were differentiated into erythrocytes by treatment with hemin (HE) and hydroxyurea (HU) for 4 days, while controls were not differentiated. RNA was then extracted for RT-qPCR using SYBR green. PIPKIIα expression relative to GAPDH was compared between groups. The primary outcome of this replication will be the comparison of relative expression levels of PIPKIIα and GAPDH between erythrocytes differentiated by hydroxyurea and hemin treatment and undifferentiated cells (non-treated cells) (n = 4/ group).* Registered protocols: [LAB39][549], [LAB48][550], [LAB56][551] Post hoc protocol amendments: [LAB39][552], [LAB48][553], [LAB56][554] Full datasets: [LAB39][555], [LAB48][556], [LAB56][557] Processed datasets: [LAB39][558], [LAB48][559], [LAB56][560] ([LABUNIT][561]) Scatter plots: [LAB39][562], [LAB48][563] **PCR147** *MCF-10A and MDA-MB-435 cells were cultured in MEBM until 80% confluence. To compare the two cell lines, RNA was then extracted using RNeasy kit and qRT-PCR was performed using SYBR Green. CD90 expression relative to GAPDH and HPRT was compared between cell lines. The primary outcome of this replication will be the comparison of CD90 expression relative to GAPDH and HPRT between MCF-10A and MDA-MB-435 cell lines (n = 5/group).* Registered protocols: [LAB08][564], [LAB12][565], [LAB28][566] Post hoc protocol amendments: [LAB08][567], [LAB12][568], [LAB28][569] Full datasets: [LAB08][570], [LAB12][571], [LAB28][572] Processed datasets: [LAB08][573], [LAB12][574] Scatter plots: [LAB08][575], [LAB12][576] **PCR158** *HepG2 cells were maintained in DMEM + 10% FBS and treated for 24h with atorvastatin (1μM) or vehicle. After treatment, they were analyzed by RT-qPCR using TaqMan probes. ABCB1 expression relative to GAPDH was compared between vehicle and atorvastatin-treated cells. The primary outcome of this replication will be the comparison of relative expression levels of ABCB1 and GAPDH between atorvastatin HepG2 treated and non-treated cells (vehicle) (n = 5/ group).* Registered protocols: [LAB48][577], [LAB56][578], [LAB89][579] Post hoc protocol amendments: [LAB48][580], [LAB56][581], [LAB89][582] Full datasets: [LAB48][583], [LAB56][584], [LAB89][585] Processed datasets: [LAB48][586], [LAB56][587] ([LABUNIT][588]), [LAB89][589] Scatter plots: [LAB48][590], [LAB89][591] **PCR16** *MES-SA/Dx5 cells were plated in 6-well plates in McCoy's 5A medium with 10% heat-inactivated FBS supplementation. After 24 h, the control group (0h) was maintained in the FBS-supplemented medium and the experimental group had its medium switched to an FBS-free medium. Both groups remained in their respective conditions for 24 h. After serum starvation, mRNA was extracted for RT-qPCR using SYBR Green for comparison with control cells (0h). The primary outcome of this replication will be the comparison of ABCB1 expression relative to β2-microglobulin (β2M) between cells in starvation (24h) and cells in no starvation (control; 0h) (n = 3/ group).* Registered protocols: [LAB22][592], [LAB43][593], [LAB56][594] Post hoc protocol amendments: [LAB22][595], [LAB43][596], [LAB56][597] Full datasets: [LAB22][598], [LAB43][599], [LAB56][600] Processed datasets: [LAB22][601], [LAB43][602], [LAB56][603] ([LABUNIT][604]) Scatter plots: [LAB22][605], [LAB43][606] ([no outliers][607]) **PCR166** *The murine GRX - Hepatic Stellate Cell line was maintained in DMEM and treated for 10 days with 100 μM of capsaicin, while controls remained untreated. Afterward, RNA was extracted for RT-qPCR using BRYTE Green. The primary outcome of this replication will be the comparison of MMP-13 expression relative to GAPDH between capsaicin-treated and non-treated cells for 10 days (n = 3/ group).* Registered protocols: [LAB32][608], [LAB36][609] Post hoc protocol amendments: [LAB32][610], [LAB36][611] Full datasets: [LAB32][612], [LAB36][613] Processed datasets: [LAB32][614], [LAB36][615] Scatter plots: [LAB32][616], [LAB36][617] **PCR175** *HaCaT cells were cultivated in DMEM and treated with either gallic acid (10 μg/ml) or left with a medium-plus vehicle (control) for 24h. After the incubation, RNA was extracted and RT-qPCR was performed using SYBR green to compare expression levels of HIF1α relative to b-actin between treated and non-treated groups. The primary outcome of this replication will be HIF-1α mRNA expression relative to β-actin compared between non-treated control cells (with the ethanol vehicle) and gallic acid-treated cells (n = 3/ group).* Registered protocols: [LAB27][618], [LAB47][619], [LAB56][620] Post hoc protocol amendments: [LAB27][621], [LAB56][622] Full datasets: [LAB27][623], [LAB56][624] Processed datasets: [LAB27][625] ([LABUNIT][626]), [LAB56][627] ([LABUNIT][628]) Scatter plots: -- **PCR184** *Jurkat cells were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum. Cells were resuspended at a density of 2 x 10 5 cells/mL in 25cm3 flasks and treated for 24 hours with docosahexaenoic acid (DHA) at 12.5 μmol/L, while controls were treated with ethanol. Conventional RT-PCR was performed and analyzed in a 1.5% agarose gel with ethidium bromide. MAPKK3 expression relative to actin was compared between vehicle and DHA-treated cells. The outcome of this replication will be the comparison of MAPKK3 relative to ACTB levels between Jurkat DHA-treated cells and vehicle-treated cells (n = 7/group).* Registered protocols: [LAB28][629], [LAB35][630], [LAB66][631] Post hoc protocol amendments: [LAB28][632], [LAB35][633], [LAB66][634] Full datasets: [LAB28][635], [LAB35][636], [LAB66][637] Processed datasets: [LAB28][638], [LAB35][639], [LAB66][640] Scatter plots: [LAB28][641] ([no outliers][642]), [LAB35][643] **PCR23** *Hypoxia was induced in T24 bladder cancer cells using AnaeroGenCompact foil sachets in sealed plastic bags for 8h. After treatment, RNA was extracted for RT-qPCR using SYBR green. The primary outcome of this replication will be the comparison of VEFG-A expression relative to TBP between cells that undergo hypoxia and non-hypoxic cells (n = 8/group).* Registered protocols: [LAB36][644], [LAB39][645] Post hoc protocol amendments: [LAB36][646], [LAB39][647] Full datasets: [LAB36][648], [LAB39][649] Processed datasets: [LAB36][650], [LAB39][651] Scatter plots: [LAB36][652] ([no outliers][653]), [LAB39][654] ([no outliers][655]) **PCR29** *The U-87MG human malignant glioblastoma cell line was cultured in DMEM and treated with glutamate for 48h. After that, RNA was extracted for semi-quantitative conventional RT-PCR for comparison with non-glutamate-treated cells. PCR products were analyzed in 2% agarose gel. The primary outcome of this replication will be EGFR level expression relative to GAPDH between glutamate-treated and non-treated control cells (n = 8/group).* Registered protocols: [LAB22][656], [LAB28][657], [LAB32][658] Post hoc protocol amendments: [LAB22][659], [LAB28][660], [LAB32][661] Full datasets: [LAB22][662], [LAB28][663], [LAB32][664] Processed datasets: [LAB22][665], [LAB32][666] Scatter plots: [LAB22][667], [LAB32][668] ([no outliers][669]) **PCR35** *Cultures of the Ma104 cell line were incubated with ouabain or in medium only for 24 hours. Semi-quantitative RT-PCR analysis was then performed and results were analyzed by electrophoresis in 2% agarose gel with visualization using ethidium bromide under ultraviolet light. A BCC1 expression relative to b-actin was compared between control and ouabain treated cells (n = 7/group).* Registered protocols: [LAB23][670], [LAB28][671], [LAB66][672] Post hoc protocol amendments: [LAB23][673], [LAB28][674], [LAB66][675] Full datasets: [LAB23][676], [LAB28][677], [LAB66][678] Processed datasets: [LAB23][679] ([LABUNIT][680]), [LAB28][681], [LAB66][682] Scatter plots: [LAB23][683], [LAB28][684] **PCR40** *3-month-old female C57Bl/6 mice were treated daily with 50mg/kg of caffeine diluted in filtered water by gavage for 15 days, while the control group received only filtered water. After treatment, mice were euthanized with thiopental, and liver samples were collected and frozen for RT-qPCR using TaqMan probes. The primary outcome of this replication will be the comparison of Nr1i3 expression relative to 18s between vehicle and caffeine-treated animals (n = 6/ group).* Registered protocols: [LAB34][685], [LAB39][686], [LAB55][687] Post hoc protocol amendments: [LAB34][688], [LAB39][689], [LAB55][690] Full datasets: [LAB34][691], [LAB39][692], [LAB55][693] Processed datasets: [LAB34][694], [LAB39][695], [LAB55][696] Scatter plots: [LAB34][697], [LAB39][698], [LAB55][699] ([no outliers][700]) **PCR42** *U138MG cell lines were cultured for 2 or 6 days. Conventional semi-quantitative RT-PCR was then performed and quantified in agarose gel with visualization using ethidium bromide under ultraviolet light. The primary outcome of this replication will be Ecto-5’-NT/CD73 mRNA expression levels relative to GAPDH compared between cells at sub-confluence (~50%, 2 days culture group) and confluence (100%, 6 days culture group), n = 5/group.* Registered protocols: [LAB23][701], [LAB41][702], [LAB90][703] Post hoc protocol amendments: [LAB23][704], [LAB41][705], [LAB90][706] Full datasets: [LAB23][707], [LAB41][708], [LAB90][709] Processed datasets: [LAB23][710] ([LABUNIT][711]), [LAB41][712], [LAB90][713] Scatter plots: [LAB23][714], [LAB41][715] ([no outliers][716]), [LAB90][717] ([no outliers][718]) **PCR61** *The murine GRX-Hepatic Stellate Cell line was maintained in DMEM + 5% FBS and treated for 12h with 5uM All-trans-retinol (RHO) in addition to 0.1uM Resveratrol (RSV) or vehicle. Then mRNA was extracted for RT-qPCR using HOT FIREPol® EvaGreen®. PPAR-y expression relative to B-actin was compared between cells treated with RHO+RSV and RHO only (n = 3/group). The primary outcome of this replication will be the PPAR-y mRNA levels relative to B-actin compared in 5uM RHO + 0.1uM RSV and 5uM RHO only treated GRX cells for 12 hours.* Registered protocols: [LAB14][719], [LAB32][720], [LAB43][721] Post hoc protocol amendments: [LAB14][722], [LAB32][723], [LAB43][724] Full datasets: [LAB14][725], [LAB32][726], LAB43 ([LINK1][727], [LINK2][728]) Processed datasets: [LAB14][729], [LAB32][730], [LAB43][731] Scatter plots: [LAB14][732], [LAB32][733], [LAB43][734] **PCR69** *Cultures of the GM07492 cell line were maintained in Ham-F10 + DMEM. They were treated with silver nanoparticles for 24h. Nanoparticles were suspended in a complete cell culture medium supplemented with 10% FBS at a final concentration of 100 μg/ml and sonicated by an ultrasound bath for 15 min immediately prior to culture treatments. Control cultures were kept in a medium without treatment. After incubation, RT-qPCR was performed using Taqman probes. The primary outcome of this replication will be the comparison of GADD45alpha expression relative to GUSB between silver nanoparticles treated cells and non-treated cells (controls) (n = 3/group).* Registered protocols: [LAB08][735], [LAB27][736], [LAB41][737] Post hoc protocol amendments: [LAB08][738], [LAB27][739], [LAB41][740] Full datasets: [LAB08][741], [LAB27][742], [LAB41][743] Processed datasets: [LAB08][744], [LAB27][745] ([LABUNIT][746]), [LAB41][747] Scatter plots: [LAB08][748], [LAB41][749] ([no outliers][750]) **PCR79** *MCF-7 cells, female breast cancer cell line, were cultivated in RPMI + 10% FBS and in RPMI with stripped serum (5% dextran-coated charcoal-treated FBS) to remove hormones and growth factors, for 48h. After this, they were treated with either 17-β estradiol (10 nM) or left in the same medium for 2h. Then, RNA was extracted and RT-qPCR was performed using HOT FIREPol® EvaGreen®. PAR-4 expression was compared between untreated and 17-β estradiol treated cells, relative to GAPDH expression (n=3/group). The primary outcome of this replication will be the comparison of PAR-4 mRNA levels relative to GAPDH between untreated (control 5% dextran-coated, charcoal-treated FBS-stripped serum with the vehicle) and 10nM 17-β estradiol treated cells for 2h (in the same stripped condition).* Registered protocols: [LAB14][751], [LAB34][752], [LAB47][753] Post hoc protocol amendments: [LAB14][754], [LAB34][755] Full datasets: [LAB14][756], [LAB34][757] Processed datasets: [LAB14][758], [LAB34][759] Scatter plots: [LAB14][760] ([no outliers][761]), [LAB34][762] ([no outliers][763]) [1]: https://osf.io/98cv2 [2]: https://osf.io/xdj8h [3]: https://osf.io/2mxd5 [4]: https://osf.io/anhtk [5]: https://osf.io/27d54 [6]: https://osf.io/89jwe [7]: https://osf.io/csjgw [8]: https://osf.io/369q2 [9]: https://osf.io/ghyzc/ [10]: https://osf.io/k4f96 [11]: https://osf.io/j7c29 [12]: https://osf.io/a9zg8 [13]: https://osf.io/ztbc3 [14]: https://osf.io/g5wh9 [15]: https://osf.io/ghy9r [16]: https://osf.io/q82c5 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