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<h2>Registered report: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs</h2> <p><br> <strong>Abstract:</strong></p> <p>The <a href="https://osf.io/e81xl/wiki/home/" rel="nofollow">Reproducibility Project: Cancer Biology</a> seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs’ by Sugahara and colleagues, published in <em>Science</em> in 2010 (<a href="http://science.sciencemag.org/content/328/5981/1031" rel="nofollow">Sugahara et al., 2010</a>). The key experiments being replicated include Figure 2 and Supplemental Figure 9A. In Figure 2, Sugahara and colleagues presented data on the tumor penetrance of doxorubicin (DOX) when co-administered with the peptide iRGD, as well as the effect of co-treatment of DOX and iRGD on tumor weight and cell death. In Supplemental Figure 9A, they tracked body weight of mice treated with DOX and iRGD to provide evidence that iRGD does not increase known DOX toxicity. The Reproducibility Project: Cancer Biology is a collaboration between the <a href="https://cos.io/" rel="nofollow">Center for Open Science</a> and <a href="https://www.scienceexchange.com/" rel="nofollow">Science Exchange</a>, and the results of the replications will be published by <a href="https://elifesciences.org" rel="nofollow"><em>eLife</em></a>.</p> <hr> <h3>Contents</h3> <p>This component contains additional materials relating to the Registered Report for Study 15: Sugahara et al., 2010 (<em>Scienc</em>e).</p> <p>The published Registered Report can be found <a href="https://elifesciences.org/content/4/e06959" rel="nofollow">here</a>. </p> <p><strong>Registered Report Initial Draft</strong>:</p> <ul> <li>This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input.<ul> <li><a href="https://osf.io/u63zy/" rel="nofollow">Study 15 Registered Report initial draft</a></li> </ul> </li> </ul> <p><strong>Data Estimation</strong>:</p> <ul> <li>When original data was unable to be obtained, or original qualitative data was quantified, the estimated data is contained here. Examples include densitometric quantification of Western blot bands, or estimations of means and variances from published graphs.</li> <li><a href="https://osf.io/mipg4/" rel="nofollow">Study 15 Data Estimation</a></li> <li><a href="https://osf.io/s2jfk/" rel="nofollow">Figure 2B Graph Deconstruciton</a></li> <li><a href="https://osf.io/8apbn/" rel="nofollow">Figure 2C Graph Deconstruction</a></li> <li> <p><a href="https://osf.io/zd4yg/" rel="nofollow">Figure 2D Graph Deconstruction</a></p> </li> <li> <p>These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. Note: AUC = area under the curve.</p> <ul> <li><a href="https://osf.io/9yfr8/" rel="nofollow">Study 15 Power Calculations.xlxs</a></li> <li><a href="https://osf.io/3iwcj/" rel="nofollow">Study 15 Protocol 4 Figure 2C one-way ANOVA.R</a></li> <li><a href="https://osf.io/86wiv/" rel="nofollow">Study 15 Protocol 4 Figure S9A AUC.R</a></li> <li><a href="https://osf.io/migwd/" rel="nofollow">Study 15 Protocol 4 Figure S9A one-way ANOVA.R</a></li> <li><a href="https://osf.io/3bwyx/" rel="nofollow">Study 15 Protocol 5 Figure 2D one-way ANOVA.R</a></li> </ul> </li> </ul>
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