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<p>Target of replication: <em>A priori</em> experimental protocols, materials, and confirmatory analysis plans were peer reviewed, <a href="https://osf.io/j9bnk/" rel="nofollow">pre-registered</a>, and published by <a href="https://elifesciences.org/articles/13620" rel="nofollow"><em>eLife</em></a>.</p> <p>This component includes all primary and QC data for the replication of <a href="https://www.nature.com/articles/nature11005" rel="nofollow">Garnett et al. 2012</a>. This experiment analyzedOlaparib sensitivity after depletion of EWS-FLI1 from A673 cells.</p> <h3>Contents</h3> <p><a href="https://osf.io/dgp8q/wiki/home/" rel="nofollow">Figures component</a> contains a visualization of the results. These figures are the same as can be found in the Replication Study and include: - <strong>Figure 3. Olaparib sensitivity after depletion of EWS-FLI1 from A673 cells</strong>, which displays the results of cell viability assays performed with the indicated doses of olaparib, or an equivalent volume of vehicle control (DMSO). - <strong>Figure 3 - figure supplement 1. Cell viability assays for each biological repeat</strong>, whicih displays the same experiment as in Figure 3 with line graph of each biological repeat is plotted individually. </p> <p><a href="https://osf.io/ryvmu/wiki/home/" rel="nofollow">Data Component</a>, contains data generated from the experimental work. Csv files containing the data used in the analysis of protocol 2, which assesses Olaparib sensitivity after depletion of EWS-FLI1 from A673 cells, can be found <a href="https://osf.io/bmfaz/" rel="nofollow">here</a> for the data used in analysis for protocol 3, and <a href="https://osf.io/rdya2/" rel="nofollow">here</a> for the qPCR data used in analysis.</p> <p><a href="https://osf.io/gy6vy/" rel="nofollow">Statistical Analysis Component</a>, contains the R scripts used for the analysis.</p> <p><a href="https://osf.io/euynr/wiki/home/" rel="nofollow">Methods Component</a> includes all methods, information about equipment used, and lab notes necessary for completing this protocol.</p> <p><a href="https://osf.io/x4zwb/wiki/home/" rel="nofollow">Cell line quality Control data</a> includes results confirming the cell line was free of mycoplasma contamination as well as STR DNA profiling.</p>
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