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- Megan Showalter
- Jason Hatakeyama
- Tomas Cajka
- Kacey Vandervorst
- Kermit L. Carraway III
- Oliver Fiehn
- Alexandria Denis
- Timothy M. Errington
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Figure 3. 2HG levels in AML patient samples
AML patient peripheral blood or bone marrow samples were analyzed for intracellular metabolites. Cells had organic acids extracted, purified, and derivatized with MTBSTFA before analysis by GC-MS. Patient samples were prescreened for IDH genotypic status prior to metabolite analysis. (A) Quantitation of 2HG signal intensity relative to glutamate was dete…
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