Mitotracker Green FM (Invitrogen) (MTG) or Mitotracker Red (MTR)

doi:10.17605/OSF.IO/2GUHZ

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Mitotracker Green FM (Invitrogen) (MTG) or Mitotracker Red (MTR) are widely used for staining mitochondria of live cells depending, respectively, on the organelle lipid content (mitochondrial mass) and oxidative activity (mitochondrial membrane potential). Briefly, SH-SY5Y cells were washed on PBS saline and harvested using trypsin. Then, cells were resuspended and incubated for 20 min in the dark with 100 nM of MTG and MTR diluted into (37 °C) pre-warmed DMEM F-12. The fluorescence intensity mean of FL1-H and FL3-H was evaluated to estimate mitochondrial mass and potential, respectively

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