Complete documentation on how to run SuperCRUNCH is available at: https://github.com/dportik/SuperCRUNCH/wiki
The following generalized commands were used to run the analysis. Please note that the `/Path/To/` should be changed to the actual file locations.
This analysis ensured all sequences were oriented in the correct direction before multiple sequence alignment. This was performed in two pieces, one analysis for the nuclear markers, and one that included the mtDNA markers.
```
python Adjust_Direction.py -i /Path/To/7-Adjust-Direction/input-nucdna -o /Path/To/7-Adjust-Direction/output-nucdna --threads 10
```
```
python Adjust_Direction.py -i /Path/To/7-Adjust-Direction/input-mtdna -o /Path/To/7-Adjust-Direction/output-mtdna --threads 10
```